Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,374,259	A→G	E57G (GAG→GGG)	ycjP →	putative ABC transporter membrane subunit YcjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,374,259	0	A	G	100.0%	95.1 / NA	29	E57G (GAG→GGG)	ycjP	putative ABC transporter membrane subunit YcjP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

TGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGT  >  NC_000913/1374181‑1374330                                                                               |                                                                        tGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGc                                                                       <  1:264264/81‑1 (MQ=255)   cTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCa                                                                      <  1:264265/80‑1 (MQ=255)     gatgaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTa                                                                   >  2:264342/1‑81 (MQ=255)      atgaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTAc                                                                  <  2:264266/81‑1 (MQ=255)        gaCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGt                                                                >  2:264299/1‑81 (MQ=255)         aCCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTc                                                               <  1:264267/81‑1 (MQ=255)            tCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGa                                                             >  2:264308/1‑80 (MQ=255)                tCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACAttt                                                        >  2:264301/1‑81 (MQ=255)                  aaGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACAttttt                                                      <  2:264268/81‑1 (MQ=255)                     ggCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAAc                                                   >  2:264328/1‑81 (MQ=255)                        gcgAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAAccc                                                 >  2:264305/1‑80 (MQ=255)                           aaaGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCgatg                                             <  2:264269/81‑1 (MQ=255)                           aaaGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCgatg                                             <  2:264270/81‑1 (MQ=255)                            aaGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCgatga                                            <  2:264271/81‑1 (MQ=255)                               agGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGAttt                                         >  2:264311/1‑81 (MQ=255)                                ggCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGAtttt                                        >  2:264298/1‑81 (MQ=255)                                  cGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTcc                                      <  1:264272/81‑1 (MQ=255)                                   gATATCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTccc                                     <  1:264273/81‑1 (MQ=255)                                    aTCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCg                                    >  1:264322/1‑81 (MQ=255)                                             cATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGac                           >  2:264306/1‑81 (MQ=255)                                                ccTACGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCTCGTTTGTAGactac                        >  2:264303/1‑81 (MQ=255)                                                   aCGCTGCTGCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTc                     >  2:264313/1‑81 (MQ=255)                                                      ctgctgCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCg                   >  2:264318/1‑80 (MQ=255)                                                      ctgctgCCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGt                  <  1:264274/81‑1 (MQ=255)                                                           gcCGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAg             >  2:264319/1‑81 (MQ=255)                                                             cGCAGCAGTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTc           <  1:264275/81‑1 (MQ=255)                                                                agcagTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCtgg        >  2:264323/1‑81 (MQ=255)                                                                  cagTGGACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCtggtg      <  2:264276/81‑1 (MQ=255)                                                                       gACGCTGGGGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGgtgt  <  2:264277/80‑1 (MQ=255)                                                                               |                                                                        TGCTGATGACCTCGTTCAAGGGCGCGAAAGAGGCGATCTCACTGCATCCTACGCTGCTGCCGCAGCAGTGGACGCTGGAGCATTACGTCGACATTTTTAACCCGATGATTTTCCCGTTTGTCGACTACTTCCGTAACAGTCTGGTGGTGT  >  NC_000913/1374181‑1374330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑