Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,399,433	A→G	F32L (TTC→CTC)	fnr ←	DNA‑binding transcriptional dual regulator FNR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,399,433	0	A	G	100.0%	94.9 / NA	29	F32L (TTC→CTC)	fnr	DNA‑binding transcriptional dual regulator FNR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/19);  total (10/19)

GTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTC  >  NC_000913/1399357‑1399510                                                                             |                                                                              gTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCgg                                                                            >  2:268270/1‑80 (MQ=255)  tCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGa                                                                          <  2:268230/81‑1 (MQ=255)      gCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATg                                                                        <  1:268231/79‑1 (MQ=255)       ccTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCa                                                                      >  2:268273/1‑80 (MQ=255)            cTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAgctg                                                                >  2:268274/1‑81 (MQ=255)                   ggCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGAtg                                                         >  2:268280/1‑81 (MQ=255)                          tcCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAAt                                                  >  1:268277/1‑81 (MQ=255)                                cAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTgg                                             >  1:268275/1‑80 (MQ=255)                                 aaTGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCa                                           <  1:922155/81‑1 (MQ=255)                                   tGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAAt                                         <  1:268232/81‑1 (MQ=255)                                   tGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAAt                                         <  2:268233/81‑1 (MQ=255)                                        ttatCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGAt                                     <  2:268234/80‑1 (MQ=255)                                          atCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAg                                   >  1:268272/1‑80 (MQ=255)                                           tCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGc                                  <  1:268235/80‑1 (MQ=255)                                             aaGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGcac                                >  1:268283/1‑80 (MQ=255)                                             aaGCTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGcac                                <  1:268236/80‑1 (MQ=255)                                                cTGATCAAGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAAcc                            <  1:268237/81‑1 (MQ=255)                                                      aaGCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAg                       <  1:268238/80‑1 (MQ=255)                                                        gCTCATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACt                    <  2:268239/81‑1 (MQ=255)                                                           cATGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGaa                 >  2:268278/1‑81 (MQ=255)                                                            aTGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAAt                <  2:268240/81‑1 (MQ=255)                                                            aTGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAAt                <  1:268241/81‑1 (MQ=255)                                                             tGTTCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATg               <  2:268242/81‑1 (MQ=255)                                                               ttCGTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATgcg             >  1:268306/1‑81 (MQ=255)                                                                  gTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGcc           <  2:268244/80‑1 (MQ=255)                                                                  gTTGAGTGTGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGcc           <  1:268243/80‑1 (MQ=255)                                                                       gtgtGAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATaa     <  2:268245/81‑1 (MQ=255)                                                                        tgtgAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAAt    <  1:268246/81‑1 (MQ=255)                                                                          tgAGCGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTc  <  2:268247/81‑1 (MQ=255)                                                                             |                                                                              GTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGTGAACGGGATGCAAAGCTGGCTGATGCTGCAATCCTGGCAATGGATAGCACAACCGCCAGACTGAATGCGCCGTATAATTC  >  NC_000913/1399357‑1399510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑