Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,413,532	T→A	*70L (TAA→TTA)	rcbA ←	Rac prophage; double‑strand break reduction protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,413,532	0	T	A	100.0%	50.5 / NA	15	*70L (TAA→TTA)	rcbA	Rac prophage; double‑strand break reduction protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (6/9);  total (6/9)

ATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTC  >  NC_000913/1413456‑1413605                                                                             |                                                                          aTCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTAAAtt                                                                       >  1:270255/1‑81 (MQ=255)          ggCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTAAATTTGAATACaa                                                              >  1:270259/1‑81 (MQ=255)                        ttAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAgg                                                <  1:270241/81‑1 (MQ=255)                           gACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACt                                             <  2:270242/81‑1 (MQ=255)                                 aaTTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGtt                                       <  2:270243/81‑1 (MQ=255)                                      aCGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTtctc                                   <  1:270244/80‑1 (MQ=255)                                          ccGATGTTGATTATTAATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAAt                              <  1:270245/81‑1 (MQ=255)                                            gATGTTGATTATTAATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAAtt                             >  1:270262/1‑80 (MQ=255)                                                   attattAATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAAttt                      <  1:270246/80‑1 (MQ=255)                                                      attaATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTAt                    >  2:270260/1‑79 (MQ=255)                                                       ttaATAATCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAg                 <  1:270247/81‑1 (MQ=255)                                                        taataaTCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGt                >  1:270257/1‑81 (MQ=255)                                                              tCAGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACt          >  1:270265/1‑81 (MQ=255)                                                                aGCTATGAAGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACtt         <  2:270248/80‑1 (MQ=255)                                                                       aaGTTTAAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGctc  <  2:270249/80‑1 (MQ=255)                                                                             |                                                                          ATCACCTCTGGCAGGCGCCAATGTTAGACTGAAATTGACGCCCGATGTTGATTATTAATAATCAGCTATGAAGTTTTAATTTGAATACAATGCAATTCTCGAGGACTGAAGTTTCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTC  >  NC_000913/1413456‑1413605

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑