Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,423,242	G→A	G164E (GGG→GAG)	ydaW →	Rac prophage; putative uncharacterized protein YdaW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,423,242	0	G	A	100.0%	71.9 / NA	21	G164E (GGG→GAG)	ydaW	Rac prophage; putative uncharacterized protein YdaW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/4);  total (17/4)

CGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCT  >  NC_000913/1423165‑1423316                                                                              |                                                                           cGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGgt                                                                         >  2:271490/1‑81 (MQ=255)  gATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGgtg                                                                        <  1:271483/81‑1 (MQ=255)      aTTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTaa                                                                    >  2:271491/1‑81 (MQ=255)           ccAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCAtt                                                               <  1:271484/81‑1 (MQ=255)             agcagAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTAc                                                             >  2:271492/1‑81 (MQ=255)             agcagAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTAc                                                             >  2:271497/1‑81 (MQ=255)                agagCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGt                                                          >  1:271503/1‑81 (MQ=255)                 gagCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGt                                                          >  2:271499/1‑80 (MQ=255)                     cGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATaa                                                     >  2:271500/1‑81 (MQ=255)                        aaGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGc                                                  >  2:271511/1‑81 (MQ=255)                            agcaTCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATc                                              >  1:271504/1‑81 (MQ=255)                            agcaTCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATc                                              >  2:271508/1‑81 (MQ=255)                             gcaTCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATCg                                             >  1:271498/1‑81 (MQ=255)                               aTCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATCGcc                                           >  2:271493/1‑81 (MQ=255)                                            ctctGGTTATCTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAgc                              >  1:271501/1‑81 (MQ=255)                                                      cTGCATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACaaaaa                    >  1:271517/1‑81 (MQ=255)                                                         cATCGTCGTCTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACaaaaa                    <  1:271485/78‑1 (MQ=255)                                                             gtcgtcTGCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCa               >  1:271506/1‑79 (MQ=255)                                                                    gCCTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTg       >  2:271529/1‑80 (MQ=255)                                                                      cTGTCATGAGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAg    <  1:271486/81‑1 (MQ=255)                                                                        gTCATGAGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCt  >  2:271502/1‑81 (MQ=255)                                                                              |                                                                           CGATAATTTACCAGCAGAGCCGAAAGCAGCATCGGTATCTCCGCTCTGGTTATCTGCATCGTCGTCTGCCTGTCATGGGGTGTTAATCATTACCGTGATAACGCCATCGCCTACAAAGAACAGCGCGACAAAAATGCCAGAGAACTGAAGCT  >  NC_000913/1423165‑1423316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑