| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,425,909 | A→C | I97L (ATC→CTC) | ydaY → | Rac prophage; putative uncharacterized protein YdaY |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,425,909 | 0 | A | C | 96.4% | 92.0 / ‑4.0 | 28 | I97L (ATC→CTC) | ydaY | Rac prophage; putative uncharacterized protein YdaY |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (9/18); total (9/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TGTTGAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCC > NC_000913/1425833‑1425985 | tgtTGAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCt < 1:271718/81‑1 (MQ=255) gtTGAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCtt > 2:271745/1‑81 (MQ=255) ttGAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTg > 1:271748/1‑81 (MQ=255) atatACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCAt < 2:271719/81‑1 (MQ=255) tataCGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATc < 1:271720/81‑1 (MQ=255) tataCGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATc < 1:271721/81‑1 (MQ=255) cGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGccc < 2:271722/81‑1 (MQ=255) cAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAgg < 1:271723/81‑1 (MQ=255) gAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAag < 2:271724/81‑1 (MQ=255) aTCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGagcag < 1:271725/81‑1 (MQ=255) tCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGagcagc < 1:271726/81‑1 (MQ=255) tCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGagcagc < 1:271727/81‑1 (MQ=255) tAAGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGa > 2:271761/1‑81 (MQ=255) aaGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAgg < 2:271728/79‑1 (MQ=255) aGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTGAAGAGCAGCAGAAGCAGGAt < 2:271729/80‑1 (MQ=255) aGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATa > 2:271756/1‑81 (MQ=255) aGCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATa > 2:271757/1‑81 (MQ=255) gCAGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATAc > 2:271751/1‑81 (MQ=255) aGAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGa > 2:271754/1‑81 (MQ=255) gAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGa > 2:271752/1‑80 (MQ=255) gAATCCAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGAt < 2:271730/81‑1 (MQ=255) cAGAGTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATaaaa < 2:271731/80‑1 (MQ=255) agTTGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGc < 2:271732/81‑1 (MQ=255) ttGTACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCa < 1:271733/81‑1 (MQ=255) tACTGGCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAg < 2:271734/81‑1 (MQ=255) gCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTg < 1:271736/79‑1 (MQ=255) gCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGcc > 2:271762/1‑81 (MQ=255) gCAGCTCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGc < 2:271735/80‑1 (MQ=255) | TGTTGAGGTTGCACTGGAATATACGGCAGAACGAGGGATCGCGCTGCTTAAGCAGAATCCAGAGTTGTACTGGCAGATCCTTGCAGAAGCAGCCAGCATCGCCCAGGGTAAAGAGCAGCAGAAGCAGGATACGATAAAAAAGCCATAGCTGCC > NC_000913/1425833‑1425985 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |