Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,437,807	C→T	C993Y (TGC→TAC)	ydbK ←	putative pyruvate‑flavodoxin oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,437,807	0	C	T	100.0%	135.7 / NA	38	C993Y (TGC→TAC)	ydbK	putative pyruvate‑flavodoxin oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/20);  total (18/20)

GCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATC  >  NC_000913/1437734‑1437883                                                                          |                                                                             gCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCg                                                                        >  1:273404/1‑80 (MQ=255)     tGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATcc                                                                   <  1:273363/81‑1 (MQ=255)          gCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCAc                                                              <  1:273365/81‑1 (MQ=255)          gCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCAc                                                              >  2:273409/1‑81 (MQ=255)          gCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCAc                                                              <  2:273364/81‑1 (MQ=255)          gCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCAc                                                              >  1:273406/1‑81 (MQ=255)             aaaTTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAg                                                           <  2:273366/81‑1 (MQ=255)                 ttAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATg                                                       >  2:273403/1‑81 (MQ=255)                  tAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGt                                                      >  1:273416/1‑81 (MQ=255)                   aGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGtt                                                     >  2:273408/1‑81 (MQ=255)                    gTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGtt                                                     <  1:273369/80‑1 (MQ=255)                    gTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTg                                                    <  2:273368/81‑1 (MQ=255)                    gTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTg                                                    <  2:273367/81‑1 (MQ=255)                       aCTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGAc                                                  <  1:273370/80‑1 (MQ=255)                       aCTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGAc                                                  <  2:273371/80‑1 (MQ=255)                         tGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATTCAGCACCAGAATGTCGACGtt                                               >  2:273410/1‑81 (MQ=255)                            aCCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTc                                            <  2:273372/81‑1 (MQ=255)                              ccAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCg                                          >  1:273396/1‑81 (MQ=255)                               cAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGt                                         <  1:273374/81‑1 (MQ=255)                               cAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGt                                         <  2:273373/81‑1 (MQ=255)                                aGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTc                                        <  2:273375/81‑1 (MQ=255)                                   ggTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCaaa                                     >  1:273405/1‑81 (MQ=255)                                    gtgtCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAAc                                    <  1:273376/81‑1 (MQ=255)                                     tgtCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACt                                   >  2:273423/1‑81 (MQ=255)                                       tCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCa                                 <  1:273377/81‑1 (MQ=255)                                         gCTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCaa                                >  2:273420/1‑80 (MQ=255)                                          cTTTCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATa                              <  2:273378/81‑1 (MQ=255)                                            ttCGACGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACa                            >  1:273427/1‑81 (MQ=255)                                                aCGCCTGACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGAt                        <  1:273379/81‑1 (MQ=255)                                                      gACCACCGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGAc                  >  1:273413/1‑81 (MQ=255)                                                         caccGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGc               >  2:273440/1‑81 (MQ=255)                                                          accGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGcc              <  1:273380/81‑1 (MQ=255)                                                          accGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGc               >  2:273433/1‑80 (MQ=255)                                                           ccGGTGTTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTAAATACATGATCCAGACCGCCa             <  2:273381/81‑1 (MQ=255)                                                               tgtTGGAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGc         >  2:273430/1‑81 (MQ=255)                                                                   ggAATAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGa      <  1:273382/80‑1 (MQ=255)                                                                     aaTAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGatat   >  2:273428/1‑81 (MQ=255)                                                                      aTAGTATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATc  >  1:273419/1‑81 (MQ=255)                                                                          |                                                                             GCCGTGCTCGCCAAATTTAGTTACTGCACCCAGCGGTGTCGCTTTCGACGCCTGACCACCGGTGTTGGAATAGCATTGCGTATCCAGCACCAGAATGTTGACGTTTTCCGTCAAACTCAATACATGATCCAGACCGCCAAAGCCGATATC  >  NC_000913/1437734‑1437883

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑