Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,452,256	G→A	H463Y (CAT→TAT)	paaZ ←	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,452,256	0	G	A	100.0%	99.3 / NA	28	H463Y (CAT→TAT)	paaZ	oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/13);  total (15/13)

CGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATA  >  NC_000913/1452180‑1452329                                                                             |                                                                          cGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCg                                                                       >  2:275540/1‑81 (MQ=255)  gTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCgg                                                                      >  2:275546/1‑81 (MQ=255)  gTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCg                                                                       <  2:275508/80‑1 (MQ=255)   tAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGt                                                                     >  2:275537/1‑81 (MQ=255)   tAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGt                                                                     <  2:275509/81‑1 (MQ=255)     aaCCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTgg                                                                   <  1:275510/81‑1 (MQ=255)          ccTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCt                                                              >  2:275548/1‑81 (MQ=255)          ccTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCt                                                              >  1:275551/1‑81 (MQ=255)           cTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCtt                                                             >  1:275547/1‑81 (MQ=255)              aTTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCtttt                                                           <  1:275511/80‑1 (MQ=255)              aTTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCttt                                                            <  2:275512/79‑1 (MQ=255)                tcttcACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGcc                                                        >  2:275541/1‑81 (MQ=255)                    cACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGAc                                                     <  1:275513/80‑1 (MQ=255)                     aCCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGAct                                                    <  1:275514/80‑1 (MQ=255)                      ccGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGActct                                                  >  2:275549/1‑81 (MQ=255)                       cGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGACTCtt                                                 <  2:275515/81‑1 (MQ=255)                         cctcctGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCa                                               >  1:275559/1‑81 (MQ=255)                                   gACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCt                                     >  2:275560/1‑81 (MQ=255)                                     ccAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTg                                    >  2:275555/1‑80 (MQ=255)                                            caccaTGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTCGCCGACTCTTCATTGAGGATCTGAATTCGcc                            >  2:275563/1‑81 (MQ=255)                                             accaTGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGccc                           <  1:275516/81‑1 (MQ=255)                                              ccaTGTACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCa                          >  2:275550/1‑81 (MQ=255)                                                   tACCAGTTGTGGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCg                      >  1:275554/1‑80 (MQ=255)                                                           gtgGCAGTGGGGAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGcc              <  1:275517/80‑1 (MQ=255)                                                               cAGTGGGTAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTc         <  1:275518/81‑1 (MQ=255)                                                                    gggAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCaa    <  1:275519/81‑1 (MQ=255)                                                                      gAGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATa  <  1:275520/81‑1 (MQ=255)                                                                       aGCCATACCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATa  >  1:275553/1‑80 (MQ=255)                                                                             |                                                                          CGTAAACCGCCTAATTCTTCACCGCCTCCTGCGCGACCAGGCCCACCATGTACCAGTTGTGGCAGTGGGGAGCCATGCCCGGTGGATTCTTTTGCCGACTCTTCATTGAGGATCTGAATTCGCCCATGCGTACGTGCCGCGTCGGCAATA  >  NC_000913/1452180‑1452329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑