Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,474,105	C→T	intergenic (+92/‑116)	ydbA → / → pdxI	putative outer membrane protein, N‑terminal fragment/pyridoxine 4‑dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,474,105	0	C	T	100.0%	84.8 / NA	24	intergenic (+92/‑116)	ydbA/pdxI	putative outer membrane protein, N‑terminal fragment/pyridoxine 4‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/12);  total (12/12)

GTTGCGGTTTTTTTATTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTCTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATAC  >  NC_000913/1474053‑1474181                                                     |                                                                             gTTGCGGTTTTTTTATTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAg                                                  <  1:278974/81‑1 (MQ=255) gTTGCGGTTTTTTTATTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAg                                                  >  1:278998/1‑81 (MQ=255)     cGGTTTTTTTATTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAAt                                              >  1:279007/1‑81 (MQ=255)      ggTTTTTTTATTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATc                                             <  1:278975/81‑1 (MQ=255)          tttttATTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTg                                          <  1:278977/80‑1 (MQ=255)          tttttATTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTg                                          <  2:278976/80‑1 (MQ=255)               aTTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTaca                                    >  1:279003/1‑81 (MQ=255)                  gCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCt                                 >  1:279001/1‑81 (MQ=255)                   ccGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCt                                 <  2:278978/80‑1 (MQ=255)                    cGGATGTGGTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTc                               <  2:278979/81‑1 (MQ=255)                            gTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCattatt                       >  1:279002/1‑81 (MQ=255)                            gTACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCattat                        <  1:278980/80‑1 (MQ=255)                             tACGTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCattatt                       >  1:278995/1‑80 (MQ=255)                                gTGACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTAtc                   >  2:279008/1‑81 (MQ=255)                                  gACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTAtctc                 >  1:279010/1‑81 (MQ=255)                                  gACGCGTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTAtctc                 >  2:279004/1‑81 (MQ=255)                                    cgcgTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCtt               <  1:278981/81‑1 (MQ=255)                                     gcgTTTTGTTTTGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCttt              <  1:278982/81‑1 (MQ=255)                                       gttttgttttGTGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTc            <  2:278983/81‑1 (MQ=255)                                             ttttgtGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTc       <  1:278984/80‑1 (MQ=255)                                               ttgtGTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCta     >  2:279005/1‑80 (MQ=255)                                                tgtgTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCtata   >  1:278999/1‑81 (MQ=255)                                                tgtgTTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCtat    <  2:278985/80‑1 (MQ=255)                                                 gtgtTTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATAc  >  1:279014/1‑81 (MQ=255)                                                     |                                                                             GTTGCGGTTTTTTTATTGCCGGATGTGGTACGTGACGCGTTTTGTTTTGTGTCTTTCAGGACAATAGAGCAACTCATCCAGTAATCTTGTTTACACCTTCGCATTATTTATCTCTTTTCGTTTCTATAC  >  NC_000913/1474053‑1474181

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑