| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,476,830 | T→C | Y563H (TAT→CAT) | ydbD → | DUF2773 domain‑containing protein YdbD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,476,830 | 0 | T | C | 88.2% | 39.7 / ‑0.7 | 17 | Y563H (TAT→CAT) | ydbD | DUF2773 domain‑containing protein YdbD |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (7/8); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.78e-01 | |||||||||||
GCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTAT > NC_000913/1476753‑1476899 | gCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATg < 1:279324/81‑1 (MQ=255)gCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATg < 1:279323/81‑1 (MQ=255) aCGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATATGaa < 1:279325/81‑1 (MQ=255) aCGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGa > 2:279349/1‑80 (MQ=255) tCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATATGAAAc > 2:279342/1‑80 (MQ=255) tCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAAc > 2:279340/1‑80 (MQ=255) aTGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAgc < 1:279326/81‑1 (MQ=255) aaaCAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAgcgc < 1:279327/80‑1 (MQ=255) aaaCAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCt > 2:279347/1‑81 (MQ=255) ggCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTg < 2:279328/80‑1 (MQ=255) gTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGc > 2:279354/1‑81 (MQ=255) cGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGc > 1:279348/1‑80 (MQ=255) cATCGGAAAAATAATCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCAc < 2:279329/81‑1 (MQ=255) atCCGAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTtgat < 2:279330/80‑1 (MQ=255) gAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGAc > 2:279351/1‑79 (MQ=255) gAAAGCAGATTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCa < 2:279331/81‑1 (MQ=255) aTTCAGATGCACATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTAt > 1:279358/1‑81 (MQ=255) | GCACGTCGATGAAACAAAAAATTTGGCAACGTGCTTTATCGCATCGGAAAAATAATCCGAAAGCAGATTCAGATGCATATGAAACCAGCGCTGATATGATTTTGTCTGAGCTGATTAGCCACGGCGAAGTTGATGACCAGATGCTAT > NC_000913/1476753‑1476899 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |