Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,497,760	A→C	D302A (GAT→GCT)	opgD →	glucan biosynthesis protein D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,497,760	0	A	C	100.0%	64.7 / NA	20	D302A (GAT→GCT)	opgD	glucan biosynthesis protein D
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (9/11);  total (9/11)

TGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGATCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCA  >  NC_000913/1497685‑1497834                                                                            |                                                                           tGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTc                                                                       <  2:282315/81‑1 (MQ=255)    ccAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGt                                                                    <  2:282316/81‑1 (MQ=255)          tGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCAtgt                                                              <  2:282317/81‑1 (MQ=255)              cAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCg                                                          >  2:282339/1‑81 (MQ=255)               aGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCgg                                                         >  1:282337/1‑81 (MQ=255)                 cTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCgggg                                                       <  1:282319/81‑1 (MQ=255)                 cTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCgggg                                                       <  1:282318/81‑1 (MQ=255)                    cGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGc                                                      <  2:282320/79‑1 (MQ=255)                    cGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCaa                                                    >  2:282348/1‑81 (MQ=255)                     ggTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAAc                                                   >  2:282351/1‑81 (MQ=255)                                    cGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGc                                    >  2:282346/1‑81 (MQ=255)                                         gtgCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTcc                               >  2:282342/1‑81 (MQ=255)                                          tgCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTcc                               <  2:282321/80‑1 (MQ=255)                                           gCGATACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGc                             >  2:282354/1‑81 (MQ=255)                                              aTACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGa                          <  2:282323/81‑1 (MQ=255)                                              aTACAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGa                          <  1:282322/81‑1 (MQ=255)                                                 cAATTCATCCGCAAATTCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGaat                        >  1:282350/1‑80 (MQ=255)                                                                ttCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGc        <  1:282324/81‑1 (MQ=255)                                                                 tCATGACTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTg         >  2:282362/1‑79 (MQ=255)                                                                      aCTCTGCTCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCa  <  2:282325/81‑1 (MQ=255)                                                                            |                                                                           TGACCAGTATGTTCAGCTGCGGTACTAATGAACGTCGGATGTGCGATACAATTCATCCGCAAATTCATGACTCTGATCGTCTGTCCATGTGGCGGGGCAACGGCGAGTGGATTTGCCGTCCGCTGAATAATCCGCAAAAATTGCAGTTCA  >  NC_000913/1497685‑1497834

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑