Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,512,246	T→G	V198G (GTT→GGT)	ydcS →	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,512,246	0	T	G	100.0%	88.1 / NA	26	V198G (GTT→GGT)	ydcS	putative ABC transporter periplasmic binding protein/polyhydroxybutyrate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (17/9);  total (17/9)

CGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTG  >  NC_000913/1512171‑1512319                                                                            |                                                                          cGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAg                                                                      <  1:284415/81‑1 (MQ=255) cGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAg                                                                      >  1:284445/1‑81 (MQ=255)    aCGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCa                                                                   <  1:284416/81‑1 (MQ=255)      ggCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTTCGGACGCTGCGTTGTTCGGTAAAGCCACt                                                                 >  1:284452/1‑81 (MQ=255)         aaGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAg                                                              >  2:284447/1‑81 (MQ=255)         aaGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAg                                                              >  1:284438/1‑81 (MQ=255)          agagCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGc                                                             >  1:284451/1‑81 (MQ=255)               aaTAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAg                                                        >  1:284456/1‑81 (MQ=255)               aaTAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAg                                                        >  1:284457/1‑81 (MQ=255)                  aaaGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTg                                                     >  1:284446/1‑81 (MQ=255)                   aaGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTgg                                                    <  1:284417/81‑1 (MQ=255)                     ggCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTggg                                                   >  2:284448/1‑80 (MQ=255)                        cgcgTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCAt                                                >  1:284458/1‑80 (MQ=255)                           gTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGc                                            >  1:284453/1‑81 (MQ=255)                              cAGGCTTATGATGGCCCTATCTATATTGAGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGAt                                         >  1:284474/1‑81 (MQ=255)                               aGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGAt                                         <  1:284418/80‑1 (MQ=255)                               aGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATc                                        >  2:284462/1‑81 (MQ=255)                                 gCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCg                                      >  2:284464/1‑81 (MQ=255)                                     atgatgGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTAt                                   >  1:284466/1‑80 (MQ=255)                                         tgGCCCTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACt                              >  2:284437/1‑81 (MQ=255)                                             ccTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCAcc                          <  1:284420/81‑1 (MQ=255)                                             ccTATCTATATTGCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCAcc                          <  2:284419/81‑1 (MQ=255)                                                         gCGGACGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTa               <  2:284421/80‑1 (MQ=255)                                                              cGCTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGc         <  1:284422/81‑1 (MQ=255)                                                                cTGCGTTGTTCGGTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCgg       >  1:284469/1‑81 (MQ=255)                                                                      tgttCGGTAAAGCCACTCAGCCGCGGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTg  <  1:284423/80‑1 (MQ=255)                                                                            |                                                                          CGGACGGCAAGAGCAATAAAGGCCGCGTTCAGGCTTATGATGGCCCTATCTATATTGCGGACGCTGCGTTGTTCGTTAAAGCCACTCAGCCGCAGTTGGGCATCAGCGATCCGTATCAACTCACCGAAGAACAGTACCAGGCGGTGCTG  >  NC_000913/1512171‑1512319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑