Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,530,413	A→G	V31A (GTG→GCG)	yncO ←	protein YncO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,530,413	0	A	G	100.0%	19.2 / NA	7	V31A (GTG→GCG)	yncO	protein YncO
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/4);  total (3/4)

TTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATG  >  NC_000913/1530335‑1530484                                                                               |                                                                        ttatGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAacac                                                                        >  2:287084/1‑80 (MQ=255)       tgTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACGCCAGAAag                                                                 <  1:287070/81‑1 (MQ=255)                                 aaaaTGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACGCCAGAAAGAGAAAAATATAATATACATACAGa                                         >  1:287087/1‑79 (MQ=255)                                          tttttGACTGGATTGGTAAATGGTCATAATGAACAACGCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATgaga                              <  1:287071/81‑1 (MQ=255)                                                          tAAATGGTCATAATGAACAACGCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGgggcag              >  2:287091/1‑81 (MQ=255)                                                                 tCATAATGAACAACGCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGgggcagggc           >  1:287086/1‑77 (MQ=255)                                                                   aTAATGAACAACGCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGAt      <  1:287072/80‑1 (MQ=255)                                                                      aTGAACAACGCCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATg  <  1:287073/81‑1 (MQ=255)                                                                               |                                                                        TTATGATGTTGATTATTATGATAACAATTTGAAAAATGAAGTTTTTGACTGGATTGGTAAATGGTCATAATGAACAACACCAGAAAGAGAAAAATATAAAATACATACAGAAAGACATGAGAGCAAGGGACGGGGCAGGGCAAGATCATG  >  NC_000913/1530335‑1530484

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑