Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,539,749	C→T	A948T (GCC→ACC)	narZ ←	nitrate reductase Z subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,539,749	0	C	T	100.0%	87.9 / NA	25	A948T (GCC→ACC)	narZ	nitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/16);  total (9/16)

CAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCAT  >  NC_000913/1539692‑1539826                                                          |                                                                              cAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGccc                                                          <  1:288427/79‑1 (MQ=255) cAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGccc                                                          <  1:288426/79‑1 (MQ=255)   aCATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCtgt                                                      <  2:288428/81‑1 (MQ=255)   aCATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCtgt                                                      >  1:288462/1‑81 (MQ=255)           gTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGcc                                              <  2:288429/81‑1 (MQ=255)                tttCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCAc                                         <  1:288430/81‑1 (MQ=255)                tttCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCAc                                         <  2:288431/81‑1 (MQ=255)                     ggTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTg                                    >  1:288461/1‑81 (MQ=255)                      gtgccagtgccaGAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTgg                                   <  2:288432/81‑1 (MQ=255)                           agtgccagAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTaa                              <  2:288434/81‑1 (MQ=255)                           agtgccagAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTaa                              <  2:288433/81‑1 (MQ=255)                              gccagAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAAtt                            >  1:288466/1‑80 (MQ=255)                                 agAATCACTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGt                        >  1:288459/1‑81 (MQ=255)                                       aCTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGcc                   >  2:288463/1‑80 (MQ=255)                                       aCTTCTGAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGcc                   <  2:288435/80‑1 (MQ=255)                                             gAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGaaa            >  2:288472/1‑81 (MQ=255)                                             gAAGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGaaa            >  1:288460/1‑81 (MQ=255)                                              aaGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGaa             <  2:288436/79‑1 (MQ=255)                                               aGCGTCAATGGTGGTGTCAATTAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATc          <  1:288437/81‑1 (MQ=255)                                               aGCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATc          <  1:288438/81‑1 (MQ=255)                                                gCGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCg         <  1:288439/81‑1 (MQ=255)                                                 cGTCAATGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGa        <  2:288440/81‑1 (MQ=255)                                                     aaTGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGAttt     >  2:288464/1‑80 (MQ=255)                                                     aaTGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGAttt     >  2:288465/1‑80 (MQ=255)                                                     aaTGGTGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTc    <  1:288441/81‑1 (MQ=255)                                                       tggtggTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCAt  <  2:288442/81‑1 (MQ=255)                                                          |                                                                              CAACATGACCGTTGGTTTCCGGTGCCAGTGCCAGAATCACTTCTGAAGCGTCAATGGCGGTGTCAATCAGCGGACGCCCCTGTGCCGGGCCATCACGCTTGGTGTAATTGAGTTTGCCGAGGAAATCGATTTCAT  >  NC_000913/1539692‑1539826

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑