Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,551,332	A→C	D291A (GAT→GCT)	fdnH →	formate dehydrogenase N subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,551,332	0	A	C	100.0%	85.0 / NA	25	D291A (GAT→GCT)	fdnH	formate dehydrogenase N subunit beta
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (10/15);  total (10/15)

TATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGATTT  >  NC_000913/1551255‑1551407                                                                              |                                                                            tATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGctca                                                                          >  1:290170/1‑81 (MQ=255) tATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGctc                                                                           <  2:290130/80‑1 (MQ=255)        aCTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAg                                                                   <  2:290131/81‑1 (MQ=255)             tGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAg                                                               <  1:290133/80‑1 (MQ=255)             tGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAg                                                               <  1:290132/80‑1 (MQ=255)              gCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGt                                                              <  1:290134/80‑1 (MQ=255)                      gATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATg                                                      <  1:290135/80‑1 (MQ=255)                        ttttCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGAtt                                                   <  2:290136/81‑1 (MQ=255)                                     cGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAAt                                      >  1:290173/1‑81 (MQ=255)                                       gTATTGGCCCGAATAAGGAAGTGAACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAAttt                                    <  1:290137/81‑1 (MQ=255)                                          ttGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTAtt                                 >  1:290180/1‑81 (MQ=255)                                            ggCCCGAATAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTg                                <  2:290138/80‑1 (MQ=255)                                                  aaTAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCgcgc                         >  2:290181/1‑81 (MQ=255)                                                   aTAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGcc                        >  1:290171/1‑81 (MQ=255)                                                   aTAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGcc                        <  1:290139/81‑1 (MQ=255)                                                    tAAGGAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCt                       <  2:290140/81‑1 (MQ=255)                                                       ggAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGt                     >  1:290183/1‑80 (MQ=255)                                                       ggAAGTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTc                    <  1:290141/81‑1 (MQ=255)                                                           gTGGACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTg                <  1:290142/81‑1 (MQ=255)                                                            tGGACGAAGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTgg               <  2:290143/81‑1 (MQ=255)                                                              gACGATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGAc             <  2:290144/81‑1 (MQ=255)                                                                 gATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCgt          >  2:290182/1‑81 (MQ=255)                                                                 gATGACGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCgt          >  1:290179/1‑81 (MQ=255)                                                                     aCGAGGAGGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCgtggt       >  2:290172/1‑80 (MQ=255)                                                                       gaggagGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGAt    >  1:290188/1‑81 (MQ=255)                                                                       gaggagGCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGAt    <  1:290145/81‑1 (MQ=255)                                                                        aggaggCTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGAtt   >  2:290177/1‑81 (MQ=255)                                                                         ggaggcTCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGAttt  >  1:290169/1‑81 (MQ=255)                                                                              |                                                                            TATTGCCACTTTTGCCGGGTTGATTTTCCACTACATCGGTATTGGCCCGAATAAGGAAGTGGACGATGACGAGGAGGATCATCATGAGTAAGTCGAAAATGATTGTGCGCACCAAATTTATTGATCGCGCCTGTCACTGGACCGTGGTGATTT  >  NC_000913/1551255‑1551407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑