Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,566,656	G→A	A162V (GCC→GTC)	dosC ←	diguanylate cyclase DosC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,566,656	0	G	A	100.0%	66.1 / NA	20	A162V (GCC→GTC)	dosC	diguanylate cyclase DosC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/14);  total (6/14)

GGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAAT  >  NC_000913/1566580‑1566728                                                                             |                                                                         ggCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACact                                                                      <  2:292378/81‑1 (MQ=255)   cTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACactac                                                                    <  2:292379/81‑1 (MQ=255)      tttGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACactact                                                                   <  1:292380/79‑1 (MQ=255)         gCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCAc                                                              >  1:292401/1‑81 (MQ=255)          ccGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACt                                                             <  1:292381/81‑1 (MQ=255)                   ttcttcttcGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTaaa                                                    >  1:292410/1‑81 (MQ=255)                   ttcttcttcGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTaa                                                     <  2:292382/80‑1 (MQ=255)                     cttcttcGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTACATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAAc                                                   <  1:292383/80‑1 (MQ=255)                        cttcGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAAcgcgc                                               >  1:292409/1‑81 (MQ=255)                           cGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGa                                             <  1:292384/80‑1 (MQ=255)                            ggCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGt                                           >  2:292400/1‑81 (MQ=255)                                            gagaAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATcgcg                           <  1:292386/81‑1 (MQ=255)                                            gagaAGATACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATcgcg                           <  2:292385/81‑1 (MQ=255)                                              gaagaTACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGa                          >  2:292403/1‑80 (MQ=255)                                               aagaTACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGata                        <  1:292387/81‑1 (MQ=255)                                                   tACGATAGTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCaa                    <  1:292388/81‑1 (MQ=255)                                                          gTTTTCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGtt             <  2:292389/81‑1 (MQ=255)                                                           ttttCATCTTCCTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTa            <  2:292390/81‑1 (MQ=255)                                                                     ccTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCgag      <  1:292391/77‑1 (MQ=255)                                                                     ccTTTGAGACACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAAt  >  1:292408/1‑81 (MQ=255)                                                                             |                                                                         GGCTATTTGCCGTTCTTTTTCTTCTTCGGCGTTTTCCAGTAACGAGAAGATACGATAGTTTTCATCTTCCTTTGAGGCACTACTGTCACTAAAGGTAAACGCGCGAGTCATCACTTCCATCGCGATATCAATACTGTTAATCGAGAAAT  >  NC_000913/1566580‑1566728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑