Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,575,336	T→G	S762R (AGC→CGC)	yddB ←	putative TonB‑dependent receptor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,575,336	0	T	G	100.0%	81.6 / NA	24	S762R (AGC→CGC)	yddB	putative TonB‑dependent receptor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/9);  total (15/9)

CATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGG  >  NC_000913/1575264‑1575412                                                                         |                                                                             cATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTa                                                                      >  2:293544/1‑81 (MQ=255) cATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTa                                                                      >  1:293542/1‑81 (MQ=255) cATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTa                                                                      >  1:293547/1‑81 (MQ=255)    cAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACAt                                                                   >  2:293550/1‑81 (MQ=255)      aGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTg                                                                 <  1:293517/81‑1 (MQ=255)       gCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACAtt                                                                  >  1:293548/1‑79 (MQ=255)         cAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTgaga                                                              <  2:293518/81‑1 (MQ=255)            aaaGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAata                                                           >  1:293540/1‑81 (MQ=255)             aaGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAatat                                                          <  2:293519/81‑1 (MQ=255)                 aCGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCgg                                                       >  1:293545/1‑80 (MQ=255)                 aCGGCCACTGGCCTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCgg                                                       >  1:293559/1‑80 (MQ=255)                  cGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGc                                                      <  1:293520/80‑1 (MQ=255)                        cTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATc                                               <  1:293521/81‑1 (MQ=255)                          ggCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGt                                             >  2:293535/1‑81 (MQ=255)                                ggTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGt                                        <  2:293522/80‑1 (MQ=255)                                       acacCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgttgt                                >  2:293551/1‑81 (MQ=255)                                         acCGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTttgttgttg                              >  2:293557/1‑81 (MQ=255)                                           cGGTGTTCGTTGTATCAACAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCa                            <  2:293523/81‑1 (MQ=255)                                                            aCAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAg           <  1:293524/81‑1 (MQ=255)                                                            aCAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAg           >  2:293556/1‑81 (MQ=255)                                                             cAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAgg          <  2:293525/81‑1 (MQ=255)                                                             cAGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAg           >  1:293563/1‑80 (MQ=255)                                                              aGCGGTTTTGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAgg          >  2:293571/1‑80 (MQ=255)                                                                     ttGCGATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCgg  >  1:293558/1‑81 (MQ=255)                                                                         |                                                                             CATCAAGCCAGAAAGTACGGCCACTGGCGTAGGTCGCCACACCGGTGTTCGTTGTATCAACAGCGGTTTTGCTATCCAGTACATTGAGAATATCGGCACTGATCGTCAGGTTTTGTTGTTGCAGAAATTGTGGCGTCCAGGACAAGCGG  >  NC_000913/1575264‑1575412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑