Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	183,729	C→T	E123K (GAA→AAA)	yaeH ←	DUF3461 domain‑containing protein YaeH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	183,729	0	C	T	100.0%	94.5 / NA	27	E123K (GAA→AAA)	yaeH	DUF3461 domain‑containing protein YaeH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/14);  total (13/14)

AAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTAC  >  NC_000913/183668‑183803                                                              |                                                                           aaGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCg                                                         >  1:43961/1‑81 (MQ=255)    ggTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTg                                                      <  1:43927/81‑1 (MQ=255)    ggTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTg                                                      <  1:43926/81‑1 (MQ=255)     gTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGa                                                     <  1:43928/81‑1 (MQ=255)        gagGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATc                                                   <  1:43929/80‑1 (MQ=255)         agGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCtt                                                 <  1:43930/81‑1 (MQ=255)         agGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCt                                                  >  1:43949/1‑80 (MQ=255)             gAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTg                                             <  1:43931/81‑1 (MQ=255)               aTGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGt                                           >  1:43969/1‑81 (MQ=255)                 gCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTa                                         >  1:43968/1‑81 (MQ=255)                  cccGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTAc                                        <  1:43932/81‑1 (MQ=255)                       aTTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACgactg                                   <  1:43933/81‑1 (MQ=255)                          aaCCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACgactgact                                >  2:43972/1‑81 (MQ=255)                             cGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATTTCGCTGATCTTATTGGTTACGACTGACTc                               >  2:629075/1‑79 (MQ=255)                             cGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTc                               >  1:43979/1‑79 (MQ=255)                             cGTCAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCa                             <  2:43934/81‑1 (MQ=255)                                cAGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGt                          >  1:43965/1‑81 (MQ=255)                                 aGTTCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGt                          <  2:43935/80‑1 (MQ=255)                                   ttCGGGATTATTTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGAc                       >  2:43974/1‑81 (MQ=255)                                         attattTACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAgt                 >  2:43981/1‑81 (MQ=255)                                             tttACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAgtcgtc             <  2:43936/81‑1 (MQ=255)                                             tttACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAgtcgtc             <  2:43937/81‑1 (MQ=255)                                              ttACGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAgtcgtc             >  2:43983/1‑80 (MQ=255)                                                aCGCGTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAg          >  1:43970/1‑81 (MQ=255)                                                 cgcgTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAgg         <  1:43938/81‑1 (MQ=255)                                                    gTTAGTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATc      >  1:43963/1‑81 (MQ=255)                                                        gTTTTTTCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTAc  <  2:43939/81‑1 (MQ=255)                                                              |                                                                           AAGGGTAGAGGGGAATGCCCGAATTAACCGTCAGTTCGGGATTATTTACGCGTTAGTTTTTCCAGATCGGCTTCAATCTCGCTGATCTTATTGGTTACGACTGACTCCAGGTGACGTAAGTCGTCGAGGATCTTAC  >  NC_000913/183668‑183803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑