Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,585,441	G→C	A349G (GCC→GGC)	ydeP ←	putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,585,441	0	G	C	100.0%	103.4 / NA	31	A349G (GCC→GGC)	ydeP	putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/15);  total (16/15)

TGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTC  >  NC_000913/1585366‑1585517                                                                            |                                                                             tGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGAtt                                                                         <  2:294597/81‑1 (MQ=255)    tCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTtgt                                                                      >  2:294635/1‑81 (MQ=255)     cGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTtgt                                                                      <  1:294598/80‑1 (MQ=255)      gTGCTGAGTGATCCCCATTCCGTAACAGATAGTGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTgtgt                                                                    <  2:294599/81‑1 (MQ=255)      gTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTtgtg                                                                     <  2:294600/80‑1 (MQ=255)            aGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACt                                                              >  2:294638/1‑81 (MQ=255)               gATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAg                                                           >  1:294636/1‑81 (MQ=255)                aTCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGt                                                          >  1:294637/1‑81 (MQ=255)                aTCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTGAGt                                                          >  2:294655/1‑81 (MQ=255)                           gTAACAGATAATGGTGCATTCGGCAGCGGCGTATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACg                                               <  1:294601/81‑1 (MQ=255)                            tAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGt                                              >  1:294644/1‑81 (MQ=255)                              aCAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGtt                                             <  1:294603/80‑1 (MQ=255)                              aCAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTc                                            <  1:294602/81‑1 (MQ=255)                              aCAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTc                                            <  2:294604/81‑1 (MQ=255)                                 gATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGat                                         <  1:294605/81‑1 (MQ=255)                                    aaTGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATc                                      >  1:294652/1‑81 (MQ=255)                                            gTTCGGCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACt                              >  1:294646/1‑81 (MQ=255)                                                ggCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCgg                           >  2:294628/1‑80 (MQ=255)                                                ggCAGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGa                          <  2:294606/81‑1 (MQ=255)                                                   aGCGGCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAAtt                       <  1:294607/81‑1 (MQ=255)                                                       gCATATGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTgag                    >  2:294643/1‑80 (MQ=255)                                                         atatGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAAc                 >  2:294648/1‑81 (MQ=255)                                                         atatGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAAc                 >  2:294649/1‑81 (MQ=255)                                                            tGCGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGt               >  2:294642/1‑80 (MQ=255)                                                              cGTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCAc            <  1:294608/81‑1 (MQ=255)                                                               gTCAGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACg           <  2:294609/81‑1 (MQ=255)                                                                  aGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCg        >  2:294647/1‑81 (MQ=255)                                                                  aGCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTACCACTCGGAATTGAGAACGTCACGGc         <  2:294610/80‑1 (MQ=255)                                                                   gCCAGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCg        >  2:294650/1‑80 (MQ=255)                                                                      aGTTCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGc    >  1:294666/1‑81 (MQ=255)                                                                        ttCGCCGATTTGTGTCTGACTTAGTCCAGAAATACGTTAGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTc  <  2:294611/81‑1 (MQ=255)                                                                            |                                                                             TGTTCGTGCTGAGTGATCCCCATTCCGTAACAGATAATGGTGCGTTCGGCAGCGGCATATGCGTCAGCCAGTTCGGCGATTTGTGTCTGACTTAGTCCAGAAATACGTTCGATATCTTTCCACTCGGAATTGAGAACGTCACGGCGTAGCTC  >  NC_000913/1585366‑1585517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑