Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,589,322	G→T	A227D (GCT→GAT)	ydeT ←	fimbrial usher domain‑containing protein YdeT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,589,322	0	G	T	100.0%	69.4 / NA	20	A227D (GCT→GAT)	ydeT	fimbrial usher domain‑containing protein YdeT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/11);  total (9/11)

AATGGTAATATGGCATAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGAGCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAGGTGATGCCATCAGCATGAGCAATAATCCCA  >  NC_000913/1589244‑1589393                                                                               |                                                                        aaTGGTAATATGGCATAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATcc                                                                       <  2:295054/81‑1 (MQ=255)   tGGTAATATGGCATAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCtt                                                                     <  1:295056/81‑1 (MQ=255)   tGGTAATATGGCATAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCtt                                                                     <  2:295055/81‑1 (MQ=255)     gTAATATGGCATAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTaa                                                                   <  1:295057/81‑1 (MQ=255)        atatGGCATAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAAc                                                                  >  2:295077/1‑79 (MQ=255)          atGGCATAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAg                                                               >  2:295074/1‑80 (MQ=255)               aTAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCAt                                                         >  2:295073/1‑81 (MQ=255)                    cACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTgtgt                                                    >  2:295082/1‑81 (MQ=255)                     aCGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTc                                                   <  1:295058/81‑1 (MQ=255)                       gCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGc                                                 <  1:295059/81‑1 (MQ=255)                           gTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAg                                             <  2:295060/81‑1 (MQ=255)                           gTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAg                                             >  2:295081/1‑81 (MQ=255)                                     aTTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCa                                   <  2:295061/81‑1 (MQ=255)                                         cGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAg                                <  1:295062/80‑1 (MQ=255)                                          ggTCTGGTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAGGt                              >  2:295076/1‑81 (MQ=255)                                                gTTCTCTATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAGGTGATGc                         <  2:295063/80‑1 (MQ=255)                                                   ctctATTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAGGTGATGCCAt                      >  1:295083/1‑80 (MQ=255)                                                       aTTTTGACATTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAGGTGATGCCATCAGCa                 >  1:295075/1‑81 (MQ=255)                                                               aTTATCAGCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAGGTGATGCCATCAGCATGAGCaat         <  2:295064/81‑1 (MQ=255)                                                                      gCACCAGGATCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAGGTGATGCCATCAGCATGAGCAATAATCcca  >  1:295080/1‑81 (MQ=255)                                                                               |                                                                        AATGGTAATATGGCATAGCCACGCCAGTCGGTATGAATTCCGGTCTGGTTCTCTATTTTGACATTATCAGCACCAGGAGCCTTAACCAGAACCATTGTGTCGCCCAGCGGCTGTCCAAAGGTGATGCCATCAGCATGAGCAATAATCCCA  >  NC_000913/1589244‑1589393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑