Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,600,726	A→G	I172I (ATT→ATC)	lsrR ←	DNA‑binding transcriptional repressor LsrR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,600,726	0	A	G	100.0%	77.6 / NA	23	I172I (ATT→ATC)	lsrR	DNA‑binding transcriptional repressor LsrR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (17/6);  total (17/6)

CAGAACATCTTTGACGCAATTTTCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGAATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAACCGACGCCACCGGAGAGC  >  NC_000913/1600651‑1600798                                                                            |                                                                         cAGAACATCTTTGACGCAATTTCCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGaataat                                                                     <  1:296591/81‑1 (MQ=255)          tttGACGCAATTTTCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACt                                                            >  1:296625/1‑81 (MQ=255)           ttGACGCAATTTTCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTg                                                           >  1:296618/1‑81 (MQ=255)             gACGCAATTTTCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGc                                                          <  2:296592/80‑1 (MQ=255)                   aTTTTCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCAcgccgc                                                   <  1:296593/81‑1 (MQ=255)                   aTTTTCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCAcgccgc                                                   >  2:296617/1‑81 (MQ=255)                    ttttCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCcgcg                                                  >  1:296630/1‑81 (MQ=255)                         aTTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAg                                             <  2:296594/81‑1 (MQ=255)                             tttAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGc                                         >  2:296611/1‑81 (MQ=255)                              ttAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGcc                                        >  1:296615/1‑81 (MQ=255)                               tAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGcc                                        <  1:296595/80‑1 (MQ=255)                                  cGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGAt                                    >  1:296624/1‑81 (MQ=255)                                   gTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGAtt                                   >  2:296635/1‑81 (MQ=255)                                          cAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCa                            >  1:296616/1‑81 (MQ=255)                                            aTGTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCata                          >  1:296622/1‑81 (MQ=255)                                              gTCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCatat                         >  1:296620/1‑80 (MQ=255)                                               tCAGCGGAGGATGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCatat                         >  2:296623/1‑79 (MQ=255)                                                    ggaggaTGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAAcc                  >  2:296603/1‑81 (MQ=255)                                                         aTGCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAACCGACGc             >  2:296626/1‑81 (MQ=255)                                                           gCCCGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAACCGACGCCa           >  2:296621/1‑81 (MQ=255)                                                            cccGCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAACCGACGCCAc          >  1:296627/1‑81 (MQ=255)                                                               gCAACGGAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAACCGACGCCACCgg       <  1:296596/81‑1 (MQ=255)                                                                    ggAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAACCGACGCCACCGGAGAGc  >  1:296641/1‑81 (MQ=255)                                                                    ggAGCCGGGATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAACCGACGCCACCGGAGAGc  <  2:296597/81‑1 (MQ=255)                                                                            |                                                                         CAGAACATCTTTGACGCAATTTTCATTTTTTAGCGTACGGGCAATGTCAGCGGAGGATGCCCGCAACGGAGCCGGAATAATATTCACACTGCACGCCGCGTTAAGCTGCCCGATTCCCGTCATATAAGAACCGACGCCACCGGAGAGC  >  NC_000913/1600651‑1600798

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑