Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,610,589	A→G	V31A (GTT→GCT)	uxaB ←	tagaturonate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,610,589	0	A	G	100.0%	47.9 / NA	15	V31A (GTT→GCT)	uxaB	tagaturonate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/10);  total (5/10)

GACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCG  >  NC_000913/1610524‑1610662                                                                  |                                                                          gACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGa                                                            <  1:298054/81‑1 (MQ=255)  aCGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGaa                                                           <  1:298055/81‑1 (MQ=255)      acaacGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTa                                                       <  2:298056/81‑1 (MQ=255)      acaacGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTa                                                       <  2:298057/81‑1 (MQ=255)        aacGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTAcc                                                     >  2:298093/1‑81 (MQ=255)         acGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCTGGAAGTTACCt                                                    >  2:298088/1‑81 (MQ=255)          cGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCtt                                                   <  2:298058/81‑1 (MQ=255)            aCCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCa                                                 <  2:298059/81‑1 (MQ=255)                                   gtgCTCATTCAGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTg                           >  2:298086/1‑80 (MQ=255)                                             aGGAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCa                >  2:298091/1‑81 (MQ=255)                                              ggAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCAc               <  1:298060/81‑1 (MQ=255)                                              ggAGATCGATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCAc               <  1:298061/81‑1 (MQ=255)                                                     gATTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGaaa        <  2:298062/81‑1 (MQ=255)                                                      aTTTGCCAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGaaaa       >  1:298095/1‑81 (MQ=255)                                                           ccAGTCAGCAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATcgcg  <  2:298063/81‑1 (MQ=255)                                                                  |                                                                          GACGAACAACGACCACGCCAGAATTCAGATCGGTGTGCTCATTCAGGAGATCGATTTGCCAGTCAACAAAGGCGCGCAGGAAGTTACCTTCACCAAACTGAATGATACGTTCTGGATACTGTGCACCGGGAAAATCGCG  >  NC_000913/1610524‑1610662

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑