Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,637,304	C→T	I86I (ATC→ATT)	ydfO →	Qin prophage; protein YdfO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,637,304	0	C	T	100.0%	27.5 / NA	9	I86I (ATC→ATT)	ydfO	Qin prophage; protein YdfO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/4);  total (5/4)

GTGTCTGTCAAATTTTCAAAGGATAGGCATCTTATAGAAACGACCTCTAATAAGCTGAAATCCAGAGAGATCACATTTCAGGAATACAGAAGAAACCTTGCTAAAGCAGGAGTTTTTCGGTGGGTTACAAATATCCACGAAC  >  NC_000913/1637233‑1637374                                                                        |                                                                       gtgtCTGTCAAATTTTCAAAGGATAGGCATCTTATAGAAACGACCTCTAATAAGCTGAAATCCAGAGAGATTACATTTCAg                                                               >  2:301543/1‑81 (MQ=255)   gtctgtcAAATTTTCAAAGGATAGGCATCTTATAGAAACGACCTCTAATAAGCTGAAATCCAGAGAGATTACATTTCAgg                                                              >  1:301548/1‑80 (MQ=255)           aaTTTTCAAAGGATAGGCATCTTATAGAAACGACCTCTAATAAGCTGAAATCCAGAGAGATTACATTTCAGGAATACagaa                                                     <  1:301535/81‑1 (MQ=255)                                        aCGACCTCTAATAAGCTGAAATCCAGAGAGATTACATTTCAGGAATACAGAAGAAACCTTGCTAAAGCAGGAGTTTTTCgg                        <  2:301536/81‑1 (MQ=255)                                         cGACCTCTAATAAGCTGAAATCCAGAGAGATTACATTTCAGGAATACAGAAGAAACCTTGCTAAAGCAGGAGTTTTTCGGt                       >  1:301550/1‑81 (MQ=255)                                                  ataaGCTGAAATCCAGAGAGATTACATTTCAGGAATACAGAAGAAACCTTGCTAAAGCAGGAGTTTTTCGGTGGGTTACaa              <  2:301537/81‑1 (MQ=255)                                                          aaaTCCAGAGAGATTACATTTCAGGAATACAGAAGAAACCTTGCTAAAGCAGGAGTTTTTCGGTGGGTTACAAATATcc        <  1:301538/79‑1 (MQ=255)                                                            aTCCAGAGAGATTACATTTCAGGAATACAGAAGAAACCTTGCTAAAGCAGGAGTTTTTCGGTGGGTTACAAATATCCACg     >  1:301540/1‑80 (MQ=255)                                                               cAGAGAGATTACATTTCAGGAATACAGAAGAAACCTTGCTAAAGCAGGAGTTTTTCGGTGGGTTACAAATATCCACGAAc  >  1:301549/1‑80 (MQ=255)                                                                        |                                                                       GTGTCTGTCAAATTTTCAAAGGATAGGCATCTTATAGAAACGACCTCTAATAAGCTGAAATCCAGAGAGATCACATTTCAGGAATACAGAAGAAACCTTGCTAAAGCAGGAGTTTTTCGGTGGGTTACAAATATCCACGAAC  >  NC_000913/1637233‑1637374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑