Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	191,485	A→G	E210G (GAA→GGA)	tsf →	protein chain elongation factor EF‑Ts

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	191,485	0	A	G	100.0%	121.9 / NA	36	E210G (GAA→GGA)	tsf	protein chain elongation factor EF‑Ts
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/25);  total (11/25)

AAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTT  >  NC_000913/191407‑191561                                                                               |                                                                             aaGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCgaaaggaa                                                                            >  1:45722/1‑81 (MQ=255)   gACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATc                                                                          <  2:45677/81‑1 (MQ=255)      gTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCg                                                                         >  1:45736/1‑79 (MQ=255)      gTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCa                                                                       <  1:45678/81‑1 (MQ=255)        aTCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCa                                                                       >  2:45731/1‑79 (MQ=255)                     gTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTg                                                          >  1:45723/1‑79 (MQ=255)                     gTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGa                                                         <  2:45679/80‑1 (MQ=255)                        gAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGc                                                     <  2:45680/81‑1 (MQ=255)                        gAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGc                                                     >  1:45740/1‑81 (MQ=255)                         aaaaaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGcc                                                    <  2:45681/81‑1 (MQ=255)                            aaGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCa                                                 >  2:45721/1‑81 (MQ=255)                              gAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATg                                               <  2:45682/81‑1 (MQ=255)                               aaTACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATga                                              <  2:45684/81‑1 (MQ=255)                               aaTACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATga                                              <  1:45683/81‑1 (MQ=255)                                 tACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATgaag                                            <  2:45685/81‑1 (MQ=255)                                   ccAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATgaaga                                           <  2:45686/80‑1 (MQ=255)                                      ggTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAAtt                                       >  1:45725/1‑81 (MQ=255)                                          cAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCAcc                                   >  2:45735/1‑81 (MQ=255)                                             cTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGc                                >  1:45738/1‑81 (MQ=255)                                                gATATCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGa                              <  1:45687/80‑1 (MQ=255)                                                 atatCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAg                            <  1:45688/81‑1 (MQ=255)                                                  tatCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGt                           <  2:45689/81‑1 (MQ=255)                                                   atCGCGATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGtt                          >  2:45734/1‑81 (MQ=255)                                                      gcgATGCAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTtct                       >  1:45724/1‑81 (MQ=255)                                                            cAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGAcc                 <  2:45690/81‑1 (MQ=255)                                                            cAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGAcc                 <  1:45692/81‑1 (MQ=255)                                                            cAGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGAc                  <  2:45691/80‑1 (MQ=255)                                                             aGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCg                <  2:45694/81‑1 (MQ=255)                                                             aGTCTGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCg                <  1:45693/81‑1 (MQ=255)                                                                 tGGTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGt              >  1:45744/1‑79 (MQ=255)                                                                   gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTc             <  2:45695/78‑1 (MQ=255)                                                                   gTAAGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTc             <  1:100765/78‑1 (MQ=255)                                                                      aGCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGcc         <  2:45696/79‑1 (MQ=255)                                                                       gCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt      <  1:45697/81‑1 (MQ=255)                                                                       gCCGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgtt      <  2:45698/81‑1 (MQ=255)                                                                        ccGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttc     <  1:45699/81‑1 (MQ=255)                                                                         cGAAAGGAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttcg    <  1:45700/81‑1 (MQ=255)                                                                          gaaaggaaTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttcgt   >  1:45733/1‑81 (MQ=255)                                                                           aaaggaaTCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCcgttcgtt  <  2:45701/81‑1 (MQ=255)                                                                               |                                                                             AAGACGTATCCGCTGAAGTGGTAGAAAAAGAATACCAGGTACAGCTGGATATCGCGATGCAGTCTGGTAAGCCGAAAGAAATCGCAGAGAAAATGGTTGAAGGCCGCATGAAGAAATTCACCGGCGAAGTTTCTCTGACCGGTCAGCCGTTCGTT  >  NC_000913/191407‑191561

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑