Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,112	A→G	V10V (GTT→GTC)	rspA ←	mandelate racemase/muconate lactonizing enzyme family protein RspA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,112	0	A	G	100.0%	91.4 / NA	27	V10V (GTT→GTC)	rspA	mandelate racemase/muconate lactonizing enzyme family protein RspA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/17);  total (10/17)

GGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGC  >  NC_000913/1655037‑1655185                                                                            |                                                                          ggTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACaa                                                                       >  1:303608/1‑80 (MQ=255)    ggCACCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAAc                                                                   >  1:303615/1‑81 (MQ=255)     gCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACt                                                                  <  2:303581/81‑1 (MQ=255)          cccAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGc                                                             >  1:303616/1‑81 (MQ=255)            cAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCt                                                           <  1:303582/81‑1 (MQ=255)                gCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTAc                                                       <  2:303583/81‑1 (MQ=255)                 cccGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACg                                                      <  1:303584/81‑1 (MQ=255)                 cccGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACg                                                      <  2:303585/81‑1 (MQ=255)                       aTACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTc                                                <  1:303586/81‑1 (MQ=255)                        tACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAGAAACTTCGGCCTTTACGATCTTCa                                               <  2:303587/81‑1 (MQ=255)                               cTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGa                                        <  2:303588/81‑1 (MQ=255)                                 cAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGAtt                                      >  2:303621/1‑81 (MQ=255)                                  aGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTc                                     >  1:303622/1‑81 (MQ=255)                                  aGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTc                                     <  2:303589/81‑1 (MQ=255)                                   gtggtgATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTcc                                    >  2:303617/1‑81 (MQ=255)                                   gtggtgATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTcc                                    <  2:303590/81‑1 (MQ=255)                                      gtgATTTTTAATGTGACGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTg                                 <  1:303591/81‑1 (MQ=255)                                                     aCGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                  <  1:303593/81‑1 (MQ=255)                                                     aCGAAATTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGa                  <  1:303592/81‑1 (MQ=255)                                                        aaaTTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATg                >  2:303671/1‑80 (MQ=255)                                                         aaTTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCa              >  1:303619/1‑81 (MQ=255)                                                          aTTACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCAt             <  2:303594/81‑1 (MQ=255)                                                           ttACGCCCCGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATg            <  2:303595/81‑1 (MQ=255)                                                                   cGGACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTAc    <  1:303596/81‑1 (MQ=255)                                                                    ggACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACg   >  2:303623/1‑81 (MQ=255)                                                                     gACAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGc  <  1:303597/81‑1 (MQ=255)                                                                      aCAGGTGACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGc  >  1:303632/1‑80 (MQ=255)                                                                            |                                                                          GGTGGCATCCCCAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGC  >  NC_000913/1655037‑1655185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑