| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,667,359 | A→G | R402R (CGT→CGC) | mlc ← | DNA‑binding transcriptional repressor Mlc |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,667,359 | 0 | A | G | 95.2% | 65.1 / ‑6.3 | 21 | R402R (CGT→CGC) | mlc | DNA‑binding transcriptional repressor Mlc |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (12/8); minor base T (1/0); total (13/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGT > NC_000913/1667286‑1667426 | tATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATc > 1:305351/1‑80 (MQ=255) cAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTtat < 2:305337/81‑1 (MQ=255) aaaTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTtata > 2:305367/1‑81 (MQ=255) aaaTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTtata > 1:305360/1‑81 (MQ=255) aaTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTtata > 2:305358/1‑80 (MQ=255) aTTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACa < 1:305338/81‑1 (MQ=255) ttGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACAt > 2:305357/1‑81 (MQ=255) gagaTAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATcg > 2:305370/1‑81 (MQ=255) gagaTAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATcg > 2:305356/1‑81 (MQ=255) agaTAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATcg < 1:305339/80‑1 (MQ=255) gaTAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATcgcg < 2:305340/81‑1 (MQ=255) gcAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCtttt < 2:305341/81‑1 (MQ=255) aaTTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTAcc < 1:305343/81‑1 (MQ=255) aaTTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTAcc < 1:305342/81‑1 (MQ=255) taccAGTTAAAAAATGTTAACCCTGCCGCCGTCGAATCAACATAGACCCGTTATACATCGCGTCTTTTACCAGTTCAgcgc > 2:1033381/4‑81 (MQ=255) gAACAGTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAgcgc > 1:305362/1‑81 (MQ=255) gTTAAAAAATGTTAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGc > 1:305369/1‑80 (MQ=255) tAACCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTgg > 2:305359/1‑81 (MQ=255) aaCCCTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTgg < 1:305344/80‑1 (MQ=255) cccTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGt > 1:305363/1‑79 (MQ=255) ccTGCAACAGGCGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGt > 2:305364/1‑78 (MQ=255) | TATGCTTTCCTGGCCCAAATTGAGATAGCGCAAATTTTGGTAGAACAGTTAAAAAATGTTAACCCTGCAACAGACGAATCAACAAAGAACCGTTATACATCGCGTCTTTTACCAGTGCAGCGCCTGCCATCGTGCCCTGGT > NC_000913/1667286‑1667426 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |