Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,680,663	C→T	P369P (CCC→CCT)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,680,663	0	C	T	100.0%	96.2 / NA	27	P369P (CCC→CCT)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

TCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTC  >  NC_000913/1680588‑1680729                                                                            |                                                                   tCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTAttt                                                               >  1:307237/1‑81 (MQ=255)        cTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTc                                                        <  1:307185/81‑1 (MQ=255)        cTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTc                                                        >  1:307214/1‑81 (MQ=255)          cATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCgg                                                      >  1:307220/1‑81 (MQ=255)           aTCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGgc                                                     >  2:307228/1‑81 (MQ=255)           aTCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGgc                                                     >  1:307223/1‑81 (MQ=255)             cTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGgcgc                                                   >  2:307233/1‑81 (MQ=255)               ggCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGt                                                 >  2:307227/1‑81 (MQ=255)                gCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGgcgc                                                   <  1:307187/78‑1 (MQ=255)                gCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGgcgc                                                   <  2:307188/78‑1 (MQ=255)                gCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGgcg                                                    <  2:307186/77‑1 (MQ=255)                gCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGtt                                                >  2:307222/1‑81 (MQ=255)                       ggTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCt                                          >  1:307219/1‑80 (MQ=255)                       ggTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTg                                         <  1:307189/81‑1 (MQ=255)                         ttCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGaa                                       <  1:307190/81‑1 (MQ=255)                                  caacaCGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAc                              >  2:307234/1‑81 (MQ=255)                                  caacaCGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAc                              >  1:307232/1‑81 (MQ=255)                                    acacGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAAccc                            >  1:307226/1‑81 (MQ=255)                                      acGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGt                          <  2:307192/81‑1 (MQ=255)                                      acGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGt                          <  1:307191/81‑1 (MQ=255)                                              gACCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCa                  >  1:307229/1‑81 (MQ=255)                                               aCCATCGCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCAt                 <  1:307193/81‑1 (MQ=255)                                                     gCCTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCg           >  2:307244/1‑81 (MQ=255)                                                       cTCAGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGt         <  1:307194/81‑1 (MQ=255)                                                          aGAAATGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAgg      >  1:307242/1‑81 (MQ=255)                                                             aaTGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGgtgt   >  1:307238/1‑81 (MQ=255)                                                              aTGATTCTGGTGCCTTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTc  <  2:307195/81‑1 (MQ=255)                                                                            |                                                                   TCTGGTACTCATCTGGCTGACCGGTTCCGATTACAACACGTTGCTGACCATCGCCTCAGAAATGATTCTGGTGCCCTATTTCCTGGTCGGCGCGTTCTTGCTGAAAATCGCAACCCGTCCACTGCATAAAGCGGTAGGTGTC  >  NC_000913/1680588‑1680729

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑