| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,682,286 | G→A | R43Q (CGA→CAA) | rstA → | DNA‑binding transcriptional regulator RstA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,682,286 | 0 | G | A | 100.0% | 61.1 / NA | 18 | R43Q (CGA→CAA) | rstA | DNA‑binding transcriptional regulator RstA |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (12/6); total (12/6) | |||||||||||
GCCGCGTACCTGGCAAAACATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGC > NC_000913/1682210‑1682362 | gCCGCGTACCTGGCAAAACATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCaaga < 2:307424/80‑1 (MQ=255) aaaCATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGgtg > 1:307443/1‑81 (MQ=255) cAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGAcat > 2:307452/1‑80 (MQ=255) aGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGAcatca < 2:307425/81‑1 (MQ=255) tACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGc > 2:307447/1‑80 (MQ=255) aCCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTa > 1:307451/1‑81 (MQ=255) ccGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTAc < 2:307426/81‑1 (MQ=255) cGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTAc > 1:307450/1‑80 (MQ=255) gcgcgGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGa < 2:307427/81‑1 (MQ=255) cgcgGCGACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGAc > 1:307453/1‑81 (MQ=255) gACCAGGCCGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATg < 2:307428/81‑1 (MQ=255) cGAAGAAACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTg > 2:307459/1‑81 (MQ=255) agaaACCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGTACGGCATGACCATTTGTCg > 2:307448/1‑81 (MQ=255) aaaCCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGt > 2:307456/1‑80 (MQ=255) aaCCATTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGa > 1:307449/1‑81 (MQ=255) aTTTTGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTa > 1:307441/1‑81 (MQ=255) ttGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTAcgc > 2:307455/1‑81 (MQ=255) tGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTAcgc < 2:307430/80‑1 (MQ=255) tGCAAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTAcgc < 1:307429/80‑1 (MQ=255) | GCCGCGTACCTGGCAAAACATGATATGCAGGTTACCGTAGAGCCGCGCGGCGACCAGGCCGAAGAAACCATTTTGCGAGAAAATCCGGATTTGGTGTTACTCGACATCATGCTACCAGGCAAGGACGGCATGACCATTTGTCGTGATTTACGC > NC_000913/1682210‑1682362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |