Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	25,867	C→T	H14H (CAC→CAT)	fkpB →	peptidyl‑prolyl cis‑trans isomerase FkpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	25,867	0	C	T	100.0%	91.8 / NA	27	H14H (CAC→CAT)	fkpB	peptidyl‑prolyl cis‑trans isomerase FkpB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (16/11);  total (16/11)

ATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCT  >  NC_000913/25789‑25941                                                                               |                                                                           aTCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCAttt                                                                          >  2:6170/1‑81 (MQ=255)    cGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCa                                                                        <  2:6135/80‑1 (MQ=255)      gCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGc                                                                     <  2:6137/81‑1 (MQ=255)      gCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGc                                                                     <  2:6136/81‑1 (MQ=255)       cAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCt                                                                    <  2:6138/81‑1 (MQ=255)        aaaaaTCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCt                                                                    >  2:6180/1‑80 (MQ=255)          aaaTCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTaaa                                                                 <  2:6139/81‑1 (MQ=255)            aTCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAAc                                                               >  2:6209/1‑81 (MQ=255)                   aaTATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTcgacga                                                        >  1:6175/1‑81 (MQ=255)                    atatAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTACAACTCGACGAt                                                       >  1:6171/1‑81 (MQ=255)                         agagCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGca                                                   <  2:6140/80‑1 (MQ=255)                           agCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGcacca                                                >  1:6172/1‑81 (MQ=255)                                  cTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCAccgcc                                           >  2:6178/1‑79 (MQ=255)                                    gCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGt                                       >  2:6173/1‑81 (MQ=255)                                    gCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGt                                       >  1:6179/1‑81 (MQ=255)                                             aaTCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGc                               <  1:6141/80‑1 (MQ=255)                                             aaTCTGTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGc                               >  1:6189/1‑80 (MQ=255)                                                  gTACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCaacaac                         >  1:6169/1‑81 (MQ=255)                                                   tACAGATCAATATCTCCTTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACg                        >  2:1038022/1‑81 (MQ=255)                                                   tACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACg                        >  1:6176/1‑81 (MQ=255)                                                   tACAGAGCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACg                        >  1:6200/1‑81 (MQ=255)                                                        agCAATAGCGCCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTaaa                   >  2:6192/1‑81 (MQ=255)                                                                 gcCGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTg          <  2:285846/81‑1 (MQ=255)                                                                   cGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGtt        <  1:6142/81‑1 (MQ=255)                                                                   cGTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGtt        >  2:6191/1‑81 (MQ=255)                                                                    gTCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTc       >  1:6182/1‑81 (MQ=255)                                                                     tCCTGGTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTcc      <  2:6143/81‑1 (MQ=255)                                                                          gTGCATTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCt  <  1:6144/80‑1 (MQ=255)                                                                               |                                                                           ATCCGGCAAAAATCCTTAAATATAAGAGCAAACCTGCATGTCTGAATCTGTACAGAGCAATAGCGCCGTCCTGGTGCACTTCACGCTAAAACTCGACGATGGCACCACCGCCGAGTCTACCCGCAACAACGGTAAACCGGCGCTGTTCCGCCT  >  NC_000913/25789‑25941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑