Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	192,324	A→G	E157G (GAA→GGA)	pyrH →	UMP kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	192,324	0	A	G	100.0%	127.8 / NA	37	E157G (GAA→GGA)	pyrH	UMP kinase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/18);  total (19/18)

TGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCA  >  NC_000913/192246‑192397                                                                               |                                                                          tggtgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAg                                                                         <  1:45870/81‑1 (MQ=255)  ggtgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGc                                                                        >  1:45904/1‑81 (MQ=255)   gtgATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGc                                                                        <  1:45871/80‑1 (MQ=255)         ctctCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGAtgtg                                                                 >  2:45927/1‑81 (MQ=255)         ctctCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGAtgt                                                                  <  1:45872/80‑1 (MQ=255)         ctctCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGAtgt                                                                  <  2:45873/80‑1 (MQ=255)           ctcCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATgtggt                                                               >  2:45910/1‑81 (MQ=255)             ccgccgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGc                                                             >  1:45916/1‑81 (MQ=255)               gccgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCt                                                            >  1:45922/1‑80 (MQ=255)                 cgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGaa                                                         <  2:45874/81‑1 (MQ=255)                 cgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGa                                                          >  2:45918/1‑80 (MQ=255)                 cgGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGa                                                          >  1:45923/1‑80 (MQ=255)                   gTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAg                                                       >  1:45919/1‑81 (MQ=255)                      cAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCaa                                                    >  2:45911/1‑81 (MQ=255)                         gTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAAcc                                                  >  1:45917/1‑80 (MQ=255)                           aaCCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCa                                                 >  1:45920/1‑79 (MQ=255)                                 ttCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGAc                                         <  1:45875/81‑1 (MQ=255)                                 ttCTTTACCACCGACTCAGCAGCTTGCCTGCATGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGAc                                         <  2:45876/81‑1 (MQ=255)                                  tCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACg                                        >  2:45912/1‑81 (MQ=255)                                       accaccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCgtg                                   >  1:45934/1‑81 (MQ=255)                                         caccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGtt                                 <  2:45877/81‑1 (MQ=255)                                           ccGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTa                               <  2:45878/81‑1 (MQ=255)                                             gACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTAcc                             <  2:45879/81‑1 (MQ=255)                                                  agcagcTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGa                        >  2:45937/1‑81 (MQ=255)                                                     agcTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATcc                     <  2:45880/81‑1 (MQ=255)                                                     agcTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATcc                     >  1:45924/1‑81 (MQ=255)                                                      gcTTGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCg                    <  1:45881/81‑1 (MQ=255)                                                        ttGCCTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGc                  >  2:45935/1‑81 (MQ=255)                                                            cTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGaaa              <  2:45883/81‑1 (MQ=255)                                                            cTGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGaaa              <  1:45882/81‑1 (MQ=255)                                                             tGCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGa                >  2:45925/1‑78 (MQ=255)                                                              gCGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGa                >  2:45926/1‑77 (MQ=255)                                                               cGTGGTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAg             <  1:45884/79‑1 (MQ=255)                                                                   gTATCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCaa       <  1:45885/81‑1 (MQ=255)                                                                      tCGAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCg    <  2:45886/81‑1 (MQ=255)                                                                        gAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGc   >  1:45943/1‑80 (MQ=255)                                                                        gAAATTGGAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCa  <  1:45887/81‑1 (MQ=255)                                                                               |                                                                          TGGTGATCCTCTCCGCCGGTACAGGTAACCCGTTCTTTACCACCGACTCAGCAGCTTGCCTGCGTGGTATCGAAATTGAAGCCGATGTGGTGCTGAAAGCAACCAAAGTTGACGGCGTGTTTACCGCTGATCCGGCGAAAGATCCAACCGCA  >  NC_000913/192246‑192397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑