Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,719,889	T→C	V5A (GTA→GCA)	slyB →	outer membrane lipoprotein SlyB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,719,889	0	T	C	100.0%	62.5 / NA	19	V5A (GTA→GCA)	slyB	outer membrane lipoprotein SlyB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/5);  total (14/5)

TTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGTCAGGGGATGTTTATAC  >  NC_000913/1719823‑1719964                                                                   |                                                                            ttGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCaa                                                               >  1:312876/1‑81 (MQ=255)      cATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATgg                                                            >  2:312884/1‑79 (MQ=255)       aTGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAg                                                         <  2:312860/81‑1 (MQ=255)        tGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAgg                                                        >  1:312886/1‑81 (MQ=255)              aTGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGgtctgtc                                                  >  2:312895/1‑81 (MQ=255)                    gCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTg                                             <  1:312861/80‑1 (MQ=255)                     cGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGt                                            <  2:616862/80‑1 (MQ=255)                               cATGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGtt                                  <  2:312862/80‑1 (MQ=255)                                aTGGTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTta                                 <  2:312863/80‑1 (MQ=255)                                   gTGAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTtaataa                             >  1:312900/1‑81 (MQ=255)                                     gAACAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAAc                            >  2:312878/1‑80 (MQ=255)                                      aaCAGGAGATTTTTCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGa                          >  1:312904/1‑81 (MQ=255)                                               tttttCAATGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGt                  >  2:312893/1‑80 (MQ=255)                                                      aTGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGTCAGGGGAt          >  2:312894/1‑81 (MQ=255)                                                       tGATTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGTCAGGGGATg         >  1:312890/1‑81 (MQ=255)                                                         aTTAAACGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGTCAGGGGATGtt       >  1:312892/1‑81 (MQ=255)                                                            aaaCGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGTCAGGGGATGTTta     >  1:312882/1‑80 (MQ=255)                                                            aaaCGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGTCAGGGGATGTTta     >  1:312881/1‑80 (MQ=255)                                                              aCGCGCATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGTCAGGGGATGTTTATAc  >  2:312885/1‑81 (MQ=255)                                                                   |                                                                            TTGACCATGAGGGATGCTTGCGTGGCGTTTCATGGTGAACAGGAGATTTTTCAATGATTAAACGCGTATTGGTTGTTTCAATGGTAGGTCTGTCTCTTGTCGGTTGTGTTAATAACGACACCCTGTCAGGGGATGTTTATAC  >  NC_000913/1719823‑1719964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑