Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,740,391	G→T	A161S (GCG→TCG)	ydhC →	putative transporter YdhC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,740,391	0	G	T	100.0%	68.9 / NA	20	A161S (GCG→TCG)	ydhC	putative transporter YdhC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/11);  total (9/11)

CATGCCGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACG  >  NC_000913/1740315‑1740462                                                                             |                                                                        catGCCGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGAt                                                                     >  2:316099/1‑81 (MQ=255)          ggtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCAc                                                            >  1:316101/1‑81 (MQ=255)           gtggGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCAc                                                            <  1:316081/80‑1 (MQ=255)                   aTCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGc                                                   >  2:316107/1‑81 (MQ=255)                        cGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGcc                                                  <  1:316082/77‑1 (MQ=255)                         ggCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTAc                                             <  1:316083/81‑1 (MQ=255)                            aCTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCgt                                          <  1:316084/81‑1 (MQ=255)                                gCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCgtggt                                       <  1:316085/80‑1 (MQ=255)                                    ctctGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTg                                   <  2:316086/80‑1 (MQ=255)                                         ttAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCt                              <  1:316087/80‑1 (MQ=255)                                                   ggctgCTGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCt                   >  2:316116/1‑81 (MQ=255)                                                    gctgctGGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTg                  <  2:316088/81‑1 (MQ=255)                                                        ctgGTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTc              <  1:316089/81‑1 (MQ=255)                                                           gTCCATTTTTCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCaaa           >  2:316105/1‑81 (MQ=255)                                                                tttttCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCac      <  1:316090/81‑1 (MQ=255)                                                                 ttttCCTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCac      >  2:316127/1‑80 (MQ=255)                                                                     ccTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacgacg  >  2:316106/1‑80 (MQ=255)                                                                     ccTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacgacg  >  2:316110/1‑80 (MQ=255)                                                                     ccTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacgacg  >  1:316108/1‑80 (MQ=255)                                                                     ccTGGCAGTCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCacgacg  <  1:316091/80‑1 (MQ=255)                                                                             |                                                                        CATGCCGCTGGTGGGTCTATCTCCGGCACTGGCTCCTCTGTTAGGAAGCTGGCTGCTGGTCCATTTTTCCTGGCAGGCGATTTTCGCCACCCTGTTTGCCATTACCGTGGTGCTGATTCTGCCTATTTTCTGGCTCAAACCCACGACG  >  NC_000913/1740315‑1740462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑