Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,750,974	T→A	T14S (ACA→TCA)	ydhX ←	putative 4Fe‑4S ferredoxin‑type protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,750,974	0	T	A	100.0%	74.4 / NA	22	T14S (ACA→TCA)	ydhX	putative 4Fe‑4S ferredoxin‑type protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (12/10);  total (12/10)

ATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCAC  >  NC_000913/1750898‑1751050                                                                             |                                                                             aTGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATcc                                                                           >  2:317576/1‑80 (MQ=255)  tGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCa                                                                         <  2:317553/81‑1 (MQ=255)      aTACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGatcccatccc                                                                     >  1:317583/1‑81 (MQ=255)           gAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGaa                                                                >  2:317588/1‑81 (MQ=255)           gAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGaa                                                                >  1:903716/1‑81 (MQ=255)            aaCTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAAc                                                               <  1:317555/81‑1 (MQ=255)            aaCTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAAc                                                               <  2:317554/81‑1 (MQ=255)             aCTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACa                                                              <  1:317556/81‑1 (MQ=255)                   ttttCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTg                                                        >  1:317575/1‑81 (MQ=255)                     ttCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGc                                                       <  1:317557/80‑1 (MQ=255)                      tCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGt                                                     >  1:317579/1‑81 (MQ=255)                              cGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTg                                               >  2:317577/1‑79 (MQ=255)                                tgtTCGCTAACAGAGAAGACGCAGAACCAGTGAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAgg                                           <  2:317558/81‑1 (MQ=255)                                   tCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACa                                        >  1:317586/1‑81 (MQ=255)                                     gCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATc                                      >  1:317582/1‑81 (MQ=255)                                             agaagaCGCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATc                              >  1:317580/1‑81 (MQ=255)                                                    gCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAAt                       <  1:317560/81‑1 (MQ=255)                                                    gCAGAACCAGTAAAAAAGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAAt                       <  1:317559/81‑1 (MQ=255)                                                               aaaaaaGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTg             >  1:317570/1‑80 (MQ=255)                                                               aaaaaaGATTACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTg             >  2:317596/1‑80 (MQ=255)                                                                      aTTACTGATCCCATCTCCAGATCAAATTTGCGTCGACTGAAGGACAGCTGATGATAACCTAATGCAGTGGCTGAAACTGta     <  2:975382/81‑2 (MQ=255)                                                                       ttACTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTc     >  1:317598/1‑80 (MQ=255)                                                                          cTGATCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCAc  <  1:317561/80‑1 (MQ=255)                                                                             |                                                                             ATGGCATACCGAACTTCCTTTTCTTGCCTCGTGTTCGCTAACAGAGAAGACGCAGAACCAGTAAAAAAGATTACTGTTCCCATCCCCAGAACAAATTTGCGTCGAGTGAAGGACATCTGATTATCCCCTAATGCAGTGGCTGCAACTGTCCAC  >  NC_000913/1750898‑1751050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑