Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,756,965	T→A	I423N (ATC→AAC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,756,965	0	T	A	100.0%	72.9 / NA	21	I423N (ATC→AAC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/16);  total (5/16)

GCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACG  >  NC_000913/1756889‑1757039                                                                             |                                                                           gCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAacac                                                                         <  2:318380/80‑1 (MQ=255)        ccaccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACtg                                                                 <  2:318381/81‑1 (MQ=255)          accaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCTGCAGCTTGTTAAAGAGAACACTTCTACtgat                                                               >  2:318418/1‑81 (MQ=255)           ccaACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACtgatg                                                              <  2:318382/81‑1 (MQ=255)              aCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGAtt                                                           <  1:318384/81‑1 (MQ=255)              aCGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGAtt                                                           <  1:318383/81‑1 (MQ=255)               cGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGAttt                                                          <  2:318385/81‑1 (MQ=255)                gAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTc                                                         >  2:318419/1‑81 (MQ=255)                gAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTc                                                         <  1:318386/81‑1 (MQ=255)                  aaaaaCGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTa                                                       <  1:318387/81‑1 (MQ=255)                        ggCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCt                                                 <  1:318388/81‑1 (MQ=255)                        ggCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCt                                                 <  2:318389/81‑1 (MQ=255)                             atcaGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGt                                             >  1:318417/1‑80 (MQ=255)                                           aGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGctct                               <  2:318390/80‑1 (MQ=255)                                             cAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa                            <  1:318392/81‑1 (MQ=255)                                             cAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCa                            <  2:318391/81‑1 (MQ=255)                                                  gCGTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCg                       <  1:318393/81‑1 (MQ=255)                                                    gTTGTGCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGt                     <  2:318394/81‑1 (MQ=255)                                                       gtgCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCt                   <  1:318395/80‑1 (MQ=255)                                                        tgCCGCAGCTTGTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTg                  <  1:318396/80‑1 (MQ=255)                                                                   gTTAAAGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGt      >  1:318423/1‑81 (MQ=255)                                                                       aaGAGAACACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACg  >  2:318426/1‑81 (MQ=255)                                                                             |                                                                           GCACTGACCACCAACGAAAAAACGGCTCATCAGTTGGTACTGAGCAAAGGCGTTGTGCCGCAGCTTGTTAAAGAGATCACTTCTACTGATGATTTCTACCGTCTGGGTAAAGAACTGGCTCTGCAGAGCGGTCTGGCACACAAAGGTGACG  >  NC_000913/1756889‑1757039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑