Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,757,817	G→A	S303S (AGC→AGT)	ldtE ←	L,D‑transpeptidase LdtE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,757,817	0	G	A	100.0%	71.6 / NA	21	S303S (AGC→AGT)	ldtE	L,D‑transpeptidase LdtE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/10);  total (11/10)

CATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTT  >  NC_000913/1757739‑1757893                                                                               |                                                                             cATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCactact                                                                            <  2:318468/81‑1 (MQ=255) cATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCactact                                                                            >  2:318503/1‑81 (MQ=255)         cTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGaaa                                                                    >  2:318514/1‑81 (MQ=255)          ttGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACTGAAAc                                                                   >  1:318509/1‑81 (MQ=255)                     cACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTg                                                        >  2:318497/1‑81 (MQ=255)                         aTTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGccc                                                     >  1:318489/1‑80 (MQ=255)                                 cTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACa                                            <  2:318469/81‑1 (MQ=255)                                             aaGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCg                                 <  2:318470/80‑1 (MQ=255)                                             aaGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCg                                 <  2:318471/80‑1 (MQ=255)                                             aaGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGa                                >  2:318504/1‑81 (MQ=255)                                              aGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGAc                               <  2:318472/81‑1 (MQ=255)                                              aGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGAc                               <  2:318473/81‑1 (MQ=255)                                               ggCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGAc                               >  1:318511/1‑80 (MQ=255)                                               ggCGCATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGAc                               >  2:318510/1‑80 (MQ=255)                                                  gcATTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAAc                           >  2:318501/1‑81 (MQ=255)                                                    aTTGCTGGCTGCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACtt                         <  1:318474/81‑1 (MQ=255)                                                      tGCTGGCTGCGGGAGTTGCTCCACTACTCCCCGAAACCGGATACCCTGCCCGACGATACAACGCTCTATCGACTAACTTCt                       >  2:318515/1‑81 (MQ=255)                                                       gctggctgCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCt                       <  1:318475/80‑1 (MQ=255)                                                        ctggctgCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGa                     <  2:318476/81‑1 (MQ=255)                                                           gctgCGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATc                   <  1:318477/80‑1 (MQ=255)                                                               cGGGAGTTGCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACa               <  1:318478/80‑1 (MQ=255)                                                                       gCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTg      >  1:318522/1‑81 (MQ=255)                                                                       gCTCCACTACTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTg      >  1:318505/1‑81 (MQ=255)                                                                            actactCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCtt  >  1:318512/1‑80 (MQ=255)                                                                               |                                                                             CATATTCCCTTGCTCTGGTTCACCATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTT  >  NC_000913/1757739‑1757893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑