Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	208,192	T→A	S1023T (TCC→ACC)	dnaE →	DNA polymerase III subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	208,192	0	T	A	100.0%	129.2 / NA	36	S1023T (TCC→ACC)	dnaE	DNA polymerase III subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (16/20);  total (16/20)

GGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACT  >  NC_000913/208116‑208268                                                                             |                                                                             ggggCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCgg                                                                          <  1:49588/81‑1 (MQ=255) ggggCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCgg                                                                          >  1:49627/1‑81 (MQ=255) ggggCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTAT‑GGTATCTGCACGCTGGATGACCGTACCggg                                                                         >  2:49639/1‑81 (MQ=255)   ggCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGggc                                                                        <  2:49589/81‑1 (MQ=255)        gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGggcggc                                                                     <  1:49590/79‑1 (MQ=255)        gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCt                                                                    <  1:49591/80‑1 (MQ=255)        gttgttGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTg                                                                   >  2:49642/1‑81 (MQ=255)          tgttgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTgg                                                                  >  2:49624/1‑80 (MQ=255)            ttgCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGaa                                                                <  1:49592/80‑1 (MQ=255)                 gcgcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtg                                                          >  2:185174/1‑81 (MQ=255)                  cgcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgt                                                         >  1:49640/1‑81 (MQ=255)                  cgcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgt                                                         <  2:49593/81‑1 (MQ=255)                   gcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgtt                                                        <  1:49594/81‑1 (MQ=255)                   gcgGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgtt                                                        <  2:49595/81‑1 (MQ=255)                     gggTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGAtgttgt                                                      <  2:49596/81‑1 (MQ=255)                       gTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGttt                                                    <  2:556357/81‑1 (MQ=255)                          aTGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACt                                                 >  1:49638/1‑81 (MQ=255)                          aTGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACt                                                 >  2:49629/1‑81 (MQ=255)                           tGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTg                                                <  2:49597/81‑1 (MQ=255)                                   aaGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTg                                        <  1:49598/81‑1 (MQ=255)                                   aaGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTg                                        >  1:49637/1‑81 (MQ=255)                                             aTCGTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATAcc                              <  2:49599/81‑1 (MQ=255)                                                gTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAg                            <  2:49601/80‑1 (MQ=255)                                                gTATCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGc                           <  1:49600/81‑1 (MQ=255)                                                  aTCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCaa                         >  2:49645/1‑81 (MQ=255)                                                   tCGGTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAAt                        >  2:49643/1‑81 (MQ=255)                                                     ggTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAAtt                       <  2:49602/80‑1 (MQ=255)                                                      gTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGc                     <  1:49603/81‑1 (MQ=255)                                                      gTATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGc                     >  1:49646/1‑81 (MQ=255)                                                       tATCTGCACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCt                    >  2:49647/1‑81 (MQ=255)                                                             cACGCTGGATGACCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTgg                  <  1:49604/77‑1 (MQ=255)                                                                        aCCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATa    <  1:49605/80‑1 (MQ=255)                                                                        aCCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATAc   >  2:49641/1‑81 (MQ=255)                                                                        aCCGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATAc   >  1:49668/1‑81 (MQ=255)                                                                         ccGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACt  <  1:49606/81‑1 (MQ=255)                                                                          cGTACCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACt  >  1:49650/1‑80 (MQ=255)                                                                             |                                                                             GGGGCTCGTTGTTGCCGCGCGGGTTATGGTCACCAAGCGCGGCAATCGTATCGGTATCTGCACGCTGGATGACCGTTCCGGGCGGCTGGAAGTGATGTTGTTTACTGACGCCCTGGATAAATACCAGCAATTGCTGGAAAAAGACCGCATACT  >  NC_000913/208116‑208268

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑