Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,774,321	T→C	V178A (GTC→GCC)	ydiB →	shikimate dehydrogenase/quinate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,774,321	0	T	C	100.0%	65.8 / NA	20	V178A (GTC→GCC)	ydiB	shikimate dehydrogenase/quinate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/5);  total (15/5)

TTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGC  >  NC_000913/1774248‑1774397                                                                          |                                                                             tttAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACg                                                                       >  1:320965/1‑81 (MQ=255)     aCCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCa                                                                   >  1:320953/1‑81 (MQ=255)       cGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCAcc                                                                 >  1:320957/1‑81 (MQ=255)        gTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCAcc                                                                 >  2:320951/1‑80 (MQ=255)        gTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAACGAAAACACCGATTGTGCCGTCACGGTCAcc                                                                 >  2:320954/1‑80 (MQ=255)           gggATGAGTTCTTCGATAAAGCCCTCGCGTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGAtc                                                             >  2:320952/1‑81 (MQ=255)           gggATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGACTGTGCCGTCACGGTCACCGAtc                                                             >  1:320960/1‑81 (MQ=255)                gAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGcc                                                        >  2:320956/1‑81 (MQ=255)                 aGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCg                                                       <  1:320931/81‑1 (MQ=255)                            aaaGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGcc                                            >  1:320972/1‑81 (MQ=255)                             aaGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCt                                           >  1:320963/1‑81 (MQ=255)                                cccTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTg                                        >  1:320958/1‑81 (MQ=255)                                cccTCGCCTTCGCGCAGCGGGTTAAGGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAGGCCTTTg                                        <  2:320932/81‑1 (MQ=255)                                 ccTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGc                                       >  2:320964/1‑81 (MQ=255)                                              cAGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCt                          >  2:320961/1‑81 (MQ=255)                                               aGCGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCtt                         <  1:320933/81‑1 (MQ=255)                                                 cGGGTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTcc                       <  1:320934/81‑1 (MQ=255)                                                  gggTTAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTccg                      <  2:320935/81‑1 (MQ=255)                                                     ttAATGAAAACACCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTccgcc                    >  1:320968/1‑80 (MQ=255)                                                               cacCGATTGTGCCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAAc         >  2:320966/1‑81 (MQ=255)                                                                       gtgcCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGc  <  1:320936/80‑1 (MQ=255)                                                                          |                                                                             TTTAACCGTCGGGATGAGTTCTTCGATAAAGCCCTCGCCTTCGCGCAGCGGGTTAATGAAAACACCGATTGTGTCGTCACGGTCACCGATCTCGCCGATCAGCAAGCCTTTGCTGAAGCCCTGGCTTCCGCCGACATTTTAACCAATGGC  >  NC_000913/1774248‑1774397

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑