Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,779,226	C→T	A26T (GCA→ACA)	ydiP ←	putative DNA‑binding transcriptional regulator YdiP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,779,226	0	C	T	100.0%	60.6 / NA	18	A26T (GCA→ACA)	ydiP	putative DNA‑binding transcriptional regulator YdiP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/10);  total (8/10)

CTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCA  >  NC_000913/1779148‑1779284                                                                               |                                                           cTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTaa                                                          <  1:321612/81‑1 (MQ=255) cTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTaa                                                          <  2:321613/81‑1 (MQ=255)       tGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGt                                                     >  2:321637/1‑80 (MQ=255)        gACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTg                                                    >  2:321640/1‑80 (MQ=255)              aaTCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGcc                                              <  1:321615/80‑1 (MQ=255)              aaTCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCg                                             <  2:321614/81‑1 (MQ=255)                   aCTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCg                                        >  2:321641/1‑81 (MQ=255)                         tttCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTAc                                  <  2:321616/81‑1 (MQ=255)                           tCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTAcc                                 <  2:321617/80‑1 (MQ=255)                            cATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCg                                <  1:321618/80‑1 (MQ=255)                             aTTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCGGc                              >  2:321653/1‑81 (MQ=255)                                      aaCGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAg                     >  1:321643/1‑81 (MQ=255)                                            aTGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGttt                <  2:321621/80‑1 (MQ=255)                                            aTGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTc               <  2:321620/81‑1 (MQ=255)                                            aTGTCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTc               <  2:321619/81‑1 (MQ=255)                                               tCCGGACTCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACt            >  1:321651/1‑81 (MQ=255)                                                      tCCCATTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTa     >  2:321642/1‑81 (MQ=255)                                                          aTTTTGGATCATCGCTAAATGTAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCa  >  2:321645/1‑80 (MQ=255)                                                                               |                                                           CTTTCTTGACGTAAATCAACTCGGTTTCATTGTCATGAACGTGATGTCCGGACTCCCATTTTGGATCATCGCTAAATGCAAAACGTGAAAGCCGTGGCGTTTTACCGGCGACAAACAGCGTTTCACTGGCGTTATCA  >  NC_000913/1779148‑1779284

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑