Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,787,109	A→G	S2P (TCC→CCC)	ppsA ←	phosphoenolpyruvate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,787,109	0	A	G	100.0%	32.9 / NA	11	S2P (TCC→CCC)	ppsA	phosphoenolpyruvate synthetase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (4/7);  total (4/7)

CCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCAT  >  NC_000913/1787037‑1787177                                                                         |                                                                     ccccAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGaa                                                               >  2:322921/1‑80 (MQ=255)  cccAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACa                                                             <  1:322904/81‑1 (MQ=255)            tCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTtgt                                                   >  1:322922/1‑81 (MQ=255)                    attcatGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGCTGGGCATCGAACAATCCTTTTGTGATAAAt                                            >  2:322924/1‑80 (MQ=255)                       catGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGaa                                         <  1:322905/80‑1 (MQ=255)                                  ggTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGaa                              <  1:322906/80‑1 (MQ=255)                                      ataCCAAAGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAAcaca                         >  2:322925/1‑81 (MQ=255)                                           aaaGCACCAGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCt                    <  1:322907/81‑1 (MQ=255)                                                   aGCGGTGACGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCAttt            <  2:322908/81‑1 (MQ=255)                                                          aCGAGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTg     <  1:322909/81‑1 (MQ=255)                                                             aGCCATTGTTGGGCATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCat  <  1:322910/81‑1 (MQ=255)                                                                         |                                                                     CCCCAACCCTGTCTACATCATTCATGCCGAGTTGGTTATACCAAAGCACCAGCGGTGACGAGCCATTGTTGGACATCGAACAATCCTTTTGTGATAAATGAACGGTTTGAGAAACACATTTCTGCGCATTTATCTTTGCAT  >  NC_000913/1787037‑1787177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑