Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,796,866	T→C	Y360C (TAT→TGT)	pheT ←	phenylalanine‑‑tRNA ligase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,796,866	0	T	C	100.0%	91.4 / NA	27	Y360C (TAT→TGT)	pheT	phenylalanine‑‑tRNA ligase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (17/10);  total (17/10)

ACCGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGC  >  NC_000913/1796790‑1796941                                                                             |                                                                            accGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACg                                                                         <  1:324366/81‑1 (MQ=255)     cAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTga                                                                     <  2:324367/81‑1 (MQ=255)       gATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTgaga                                                                   >  1:324413/1‑81 (MQ=255)           tCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGc                                                                <  2:324368/80‑1 (MQ=255)              aTCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCg                                                            <  1:324370/81‑1 (MQ=255)              aTCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCg                                                            <  2:324369/81‑1 (MQ=255)                       cGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAgg                                                   >  2:324425/1‑81 (MQ=255)                           tCGCACGTTCCATCGCTTTGTGCTTCAGTGCCGTATCAACGCCACTCTCACAACTTTTTTACTCATCGTTATTCATGCCAt                                               >  1:1017591/1‑81 (MQ=255)                              cACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgac                                            >  2:324403/1‑81 (MQ=255)                               aCGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATgac                                            >  1:324414/1‑80 (MQ=255)                                         gCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGa                                 >  1:324402/1‑81 (MQ=255)                                         gCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGa                                 >  1:324415/1‑81 (MQ=255)                                             tgtgCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCgg                             >  1:324422/1‑81 (MQ=255)                                             tgtgCTGCAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCg                              <  1:324371/80‑1 (MQ=255)                                                gctgcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGa                          >  1:324401/1‑81 (MQ=255)                                                   gcAGTGCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGa                          >  1:324406/1‑78 (MQ=255)                                                        gCCGGATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGc                  >  2:324411/1‑81 (MQ=255)                                                           ggATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCggg               >  2:324419/1‑81 (MQ=255)                                                           ggATCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCggg               >  2:324424/1‑81 (MQ=255)                                                             aTCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCt             <  2:324372/81‑1 (MQ=255)                                                              tCAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCt             >  1:324409/1‑80 (MQ=255)                                                               cAACGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTa            >  1:324408/1‑80 (MQ=255)                                                                aaCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTaa           <  1:324373/80‑1 (MQ=255)                                                                aaCGCCACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTa            >  1:324398/1‑79 (MQ=255)                                                                     cACGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAcg     <  1:324374/81‑1 (MQ=255)                                                                      aCGCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAAcgc    >  2:324426/1‑81 (MQ=255)                                                                        gCTCACAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACgcgc  <  2:324375/81‑1 (MQ=255)                                                                             |                                                                            ACCGCAGATGTCGATCAGCAGACGGGTCGCACGTTCCATCGCTTTGTGCTGCAGTGCCGGATCAACGCCACGCTCATAACGGTGAGACGCATCGGTATGCAGGCCATGACGACGAGCACGACCGGTGATAGACAGCGGGCTAAAGAACGCGC  >  NC_000913/1796790‑1796941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑