Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,807,754	G→T	intergenic (+64/‑42)	ydiZ → / → yniA	protein YdiZ/putative kinase YniA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,807,754	0	G	T	100.0%	87.7 / NA	25	intergenic (+64/‑42)	ydiZ/yniA	protein YdiZ/putative kinase YniA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/7);  total (18/7)

AGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAG  >  NC_000913/1807676‑1807828                                                                               |                                                                           aGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTtcg                                                                          >  2:325909/1‑81 (MQ=255) aGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTtcg                                                                          >  2:325898/1‑81 (MQ=255)         aaaaTAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCtt                                                                  <  2:325885/81‑1 (MQ=255)         aaaaTAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCt                                                                   >  2:325897/1‑80 (MQ=255)               aatGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATc                                                            <  2:325886/81‑1 (MQ=255)                  ggTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTgg                                                         >  1:325906/1‑81 (MQ=255)                   gTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGc                                                        <  2:325887/81‑1 (MQ=255)                    tttttGGGCAGTAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTgg                                                         >  2:325918/1‑79 (MQ=255)                     ttttGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTgg                                                         >  1:325902/1‑78 (MQ=255)                         gggCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGacaa                                                  >  2:325927/1‑81 (MQ=255)                             aataatCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATg                                              >  2:325904/1‑81 (MQ=255)                                 atCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGgag                                          <  1:696862/81‑1 (MQ=255)                                           ggtgTGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCAtg                                >  1:325910/1‑81 (MQ=255)                                            gtgtGCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCAtgt                               >  2:325905/1‑81 (MQ=255)                                              gtgCGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTgg                             >  2:325916/1‑81 (MQ=255)                                                 cGTTAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTggcag                          >  2:325920/1‑81 (MQ=255)                                                    tAGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTggcaggc                        >  2:325919/1‑80 (MQ=255)                                                     aGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTggcaggca                       <  1:325888/80‑1 (MQ=255)                                                     aGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCaa                      >  2:325925/1‑81 (MQ=255)                                                     aGCTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCaa                      >  1:325914/1‑81 (MQ=255)                                                       cTCGTGTTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAAt                     >  2:325912/1‑80 (MQ=255)                                                          gtgtTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAgt                 >  2:325930/1‑81 (MQ=255)                                                          gtgtTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAgt                 >  1:325926/1‑81 (MQ=255)                                                           tgtTTTTACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAgtc                <  2:325889/81‑1 (MQ=255)                                                                ttACACCGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCt            <  1:325890/80‑1 (MQ=255)                                                                      cGCATTCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTgagc     >  1:325917/1‑81 (MQ=255)                                                                           tCTTTCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTgagcgag  >  2:325911/1‑79 (MQ=255)                                                                               |                                                                           AGCAACAGAAAATAAATGGTTTTTGGGCAATAATCAGTCTGTGGTGTGCGTTAGCTCGTGTTTTTACACCGCATTCTTGCGCTAACCTTATGATCTGGCAGACAACATGGGAGAGACATCATGTGGCAGGCAATCAGTCGTCTTTTGAGCGAG  >  NC_000913/1807676‑1807828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑