Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,809,987	T→C	V203A (GTA→GCA)	hxpB →	hexitol phosphatase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,809,987	0	T	C	100.0%	84.4 / NA	25	V203A (GTA→GCA)	hxpB	hexitol phosphatase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/11);  total (14/11)

GCATGATCGCCTCTAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAG  >  NC_000913/1809909‑1810061                                                                               |                                                                           gCATGATCGCCTCTAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCAt                                                                          <  1:326204/81‑1 (MQ=255)  cATGATCGCCTCTAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCAtt                                                                         <  1:326205/81‑1 (MQ=255)  cATGATCGCCTCTAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCAt                                                                          >  1:326226/1‑80 (MQ=255)           ctctAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGt                                                                <  2:326206/81‑1 (MQ=255)                 agcagcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACt                                                          <  2:326207/81‑1 (MQ=255)                    agcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACttt                                                        >  2:326241/1‑80 (MQ=255)                     gcCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCa                                                      <  1:326208/81‑1 (MQ=255)                       ccGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATc                                                    <  1:326209/81‑1 (MQ=255)                            tGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGAGCCACGATTTGCATTAGCAGACGTCAAACTTTCATCGCTGa                                               <  2:326210/81‑1 (MQ=255)                                  ccATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGaa                                          <  2:326211/80‑1 (MQ=255)                                    aTCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACt                                        >  2:326251/1‑80 (MQ=255)                                     tcgtcgTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCa                                      >  1:326248/1‑81 (MQ=255)                                       gtcgtTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCAcc                                    >  2:326250/1‑81 (MQ=255)                                           ttCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCa                                 >  1:326244/1‑80 (MQ=255)                                           ttCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCa                                 >  2:326259/1‑80 (MQ=255)                                            tCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCaa                                >  2:326247/1‑80 (MQ=255)                                                 cgcCAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGAcc                          <  1:326212/81‑1 (MQ=255)                                                    cAGAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTtc                       >  2:326252/1‑81 (MQ=255)                                                      gAAGCGCAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTtctc                     <  2:326213/81‑1 (MQ=255)                                                           gcAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATTGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTa                <  2:326214/81‑1 (MQ=255)                                                            cAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTaa               >  1:326246/1‑81 (MQ=255)                                                            cAAAATGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTaa               >  2:326257/1‑81 (MQ=255)                                                                 tGATCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGATGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGAc           >  1:326245/1‑80 (MQ=255)                                                                   aTCCACGTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAg        >  1:326265/1‑81 (MQ=255)                                                                         gTTTTGCATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAg  >  2:326249/1‑81 (MQ=255)                                                                               |                                                                           GCATGATCGCCTCTAAAGCAGCCCGCATGCGTTCCATCGTCGTTCCTGCGCCAGAAGCGCAAAATGATCCACGTTTTGTATTAGCAGACGTCAAACTTTCATCGCTGACAGAACTCACCGCAAAAGACCTTCTCGGTTAATGACCAGGGGCAG  >  NC_000913/1809909‑1810061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑