Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,943	T→C	Y347C (TAC→TGC)	astC ←	succinylornithine transaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,943	0	T	C	100.0%	105.7 / NA	31	Y347C (TAC→TGC)	astC	succinylornithine transaminase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/16);  total (15/16)

CACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAA  >  NC_000913/1830867‑1831018                                                                             |                                                                            cacGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATc                                                                         <  2:329265/81‑1 (MQ=255)  acGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCg                                                                        <  2:329266/81‑1 (MQ=255)        ccACCCGCAATCAGTACCATCACGCCTGCTTACGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTc                                                                  <  2:329267/81‑1 (MQ=255)            ccGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTa                                                              >  2:329314/1‑81 (MQ=255)               cAATCAGTAACATCACGCCTGCTTTCGACGCTTCCTGAGAGATCTGTTTCGCTAGCCCGGAGCAATCGGCATTCAGTacac                                                           <  2:329268/81‑1 (MQ=255)                 aTCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAg                                                         >  1:329298/1‑81 (MQ=255)                 aTCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAg                                                         >  2:329305/1‑81 (MQ=255)                    aGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGcc                                                       >  1:329303/1‑80 (MQ=255)                      tACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAAt                                                    >  1:329306/1‑81 (MQ=255)                        ccATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATc                                                   <  2:329269/80‑1 (MQ=255)                          aTCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAg                                                 >  1:329308/1‑80 (MQ=255)                             aCGCCTGCTTTCGCCGCTTCCTGAGAGATCTATTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCaaa                                             <  2:329270/81‑1 (MQ=255)                                        cgccgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCcgc                                   >  1:329317/1‑80 (MQ=255)                                         gccgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCcgcg                                  >  2:329313/1‑80 (MQ=255)                                          ccgcTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGaa                                <  2:329271/81‑1 (MQ=255)                                               tCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCa                           >  2:329326/1‑81 (MQ=255)                                               tCCTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCa                           >  1:329312/1‑81 (MQ=255)                                                ccTGAGAGATCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTc                            <  1:329272/79‑1 (MQ=255)                                                    agagaTCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTg                        <  2:329273/79‑1 (MQ=255)                                                    agagaTCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGaa                      >  1:329316/1‑81 (MQ=255)                                                    agagaTCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGa                       <  2:329274/80‑1 (MQ=255)                                                        aTCTGTTTCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACaaa                  >  1:329311/1‑81 (MQ=255)                                                             tttCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCAt              <  2:329275/80‑1 (MQ=255)                                                             tttCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATa             <  1:329276/81‑1 (MQ=255)                                                              ttCGCTTGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAg            >  2:329321/1‑81 (MQ=255)                                                                  cTTGCCCAGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCGCTGAACAAACCATAGCGGt        <  2:329277/81‑1 (MQ=255)                                                                    tGCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGa      >  2:329319/1‑81 (MQ=255)                                                                     gCCCGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTg       <  2:329278/79‑1 (MQ=255)                                                                      cccGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGAtt    <  1:329279/81‑1 (MQ=255)                                                                      cccGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGAt     <  2:329280/80‑1 (MQ=255)                                                                        cGGCGCAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTaa  >  2:329325/1‑81 (MQ=255)                                                                          gcgcAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTaa  >  1:329324/1‑79 (MQ=255)                                                                             |                                                                            CACGTTGCCACCCGCAATCAGTACCATCACGCCTGCTTTCGCCGCTTCCTGAGAGATCTGTTTCGCTTGCCCGGCGTAATCGGCATTCAGTACACAGCCAATCAGCAAACCTAAGCCGCGAACTTCACTGAACAAACCATAGCGGTGATTAA  >  NC_000913/1830867‑1831018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑