Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	221,181	C→T	V147V (GTG→GTA)	metI ←	L‑methionine/D‑methionine ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	221,181	0	C	T	100.0%	143.5 / NA	40	V147V (GTG→GTA)	metI	L‑methionine/D‑methionine ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (19/21);  total (19/21)

ACCTAAACCACCGGCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTCACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCACCCATTGCGC  >  NC_000913/221103‑221251                                                                               |                                                                       aCCTAAACCACCGGCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTAc                                                                      >  1:52583/1‑81 (MQ=255)        ccaccGGCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGAccc                                                               >  1:52592/1‑81 (MQ=255)         caccGGCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCg                                                              >  1:52604/1‑81 (MQ=255)           ccGGCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGc                                                            <  1:52542/81‑1 (MQ=255)             ggCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAg                                                          >  2:52598/1‑81 (MQ=255)              gCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAgc                                                         <  2:52543/81‑1 (MQ=255)              gCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAgc                                                         >  1:52618/1‑81 (MQ=255)                aCCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAgcgc                                                       >  2:52593/1‑81 (MQ=255)                  cGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCtt                                                     <  1:52544/81‑1 (MQ=255)                   gACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTc                                                    <  1:52545/81‑1 (MQ=255)                    aCTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTcc                                                   >  2:52616/1‑81 (MQ=255)                    aCTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTcc                                                   >  1:52613/1‑81 (MQ=255)                    aCTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTcc                                                   >  1:52596/1‑81 (MQ=255)                       gCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCgg                                                 >  2:52591/1‑80 (MQ=255)                        caccacCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGt                                                <  2:52546/80‑1 (MQ=255)                              ccATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCa                                         <  1:52547/81‑1 (MQ=255)                               cATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCAc                                        >  2:52615/1‑81 (MQ=255)                                aTCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCAcc                                       >  1:52607/1‑81 (MQ=255)                                    cgGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTAc                                   >  1:52594/1‑81 (MQ=255)                                    cgGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTAc                                   <  1:52548/81‑1 (MQ=255)                                      gAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACg                                  >  2:52590/1‑80 (MQ=255)                                        aTAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGAc                               <  1:52549/81‑1 (MQ=255)                                           aCCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGAt                            >  2:52608/1‑81 (MQ=255)                                           aCCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGAt                            >  1:52597/1‑81 (MQ=255)                                            ccGACCAGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATc                           <  1:52550/81‑1 (MQ=255)                                                  aGGGTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATc                     <  2:52551/81‑1 (MQ=255)                                                   gggTAATCAGGGTGATAGTTGCCGCGTTTACCAGACCCGGCAGCGCTTCCGGTAACGGCACCTTACGGACGATCTGCATCg                    <  2:52552/81‑1 (MQ=255)                                                    ggTAATCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCgg                   <  1:52553/81‑1 (MQ=255)                                                        aTCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTg               <  2:52554/81‑1 (MQ=255)                                                         tCAGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTgg              >  2:52610/1‑81 (MQ=255)                                                           aGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGc             <  2:52555/80‑1 (MQ=255)                                                           aGGGTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCa            >  2:52609/1‑81 (MQ=255)                                                              gTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCAccc         <  2:52557/81‑1 (MQ=255)                                                              gTGATAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCAccc         <  2:52556/81‑1 (MQ=255)                                                                 aTAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCACCCAtt      <  2:52558/81‑1 (MQ=255)                                                                  tAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCACCCATTg     <  1:52559/81‑1 (MQ=255)                                                                  tAGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCACCCATTg     <  1:52560/81‑1 (MQ=255)                                                                   aGTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCACCCATTgc    >  1:52630/1‑81 (MQ=255)                                                                    gTTGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCACCCATTgc    <  1:52561/80‑1 (MQ=255)                                                                     ttGCCGCATTTACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCACCCATTgcgc  <  2:52562/81‑1 (MQ=255)                                                                               |                                                                       ACCTAAACCACCGGCACCGACTGCACCACCCATCGCGGAATAACCGACCAGGGTAATCAGGGTGATAGTTGCCGCATTCACCAGACCCGGCAGCGCTTCCGGTAACAGCACCTTACGGACGATCTGCATCGGCGTGGCACCCATTGCGC  >  NC_000913/221103‑221251

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑