Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,846,373	T→C	G196G (GGA→GGG)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,846,373	0	T	C	100.0%	70.9 / NA	22	G196G (GGA→GGG)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/12);  total (10/12)

AAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAG  >  NC_000913/1846295‑1846450                                                                               |                                                                              aaaGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGccc                                                                             <  2:331699/81‑1 (MQ=255)  aagaagTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGccc                                                                             >  2:331748/1‑80 (MQ=255)      agTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACgg                                                                        <  1:331700/81‑1 (MQ=255)       gTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACgg                                                                        >  2:331745/1‑80 (MQ=255)                   gAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCt                                                            >  1:331756/1‑80 (MQ=255)                   gAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTg                                                           <  2:331701/81‑1 (MQ=255)                         ttCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAAc                                                      <  2:331702/80‑1 (MQ=255)                          tCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCg                                                    >  1:331760/1‑81 (MQ=255)                             aTCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCa                                                 >  2:331750/1‑81 (MQ=255)                                  ttCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCAtt                                               <  2:331704/78‑1 (MQ=255)                                  ttCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTg                                              <  1:331703/79‑1 (MQ=255)                                   tCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGa                                           >  2:331761/1‑81 (MQ=255)                                    cATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGc                                             <  2:331705/78‑1 (MQ=255)                                                 caccaGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGtata                             >  2:331754/1‑81 (MQ=255)                                                  accaGCCCAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATAc                            <  2:331706/81‑1 (MQ=255)                                                        ccAAGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACgg                      <  2:331707/81‑1 (MQ=255)                                                          aaGCACGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGt                    >  1:331737/1‑81 (MQ=255)                                                           aGCAGGGGCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTc                   <  1:331708/81‑1 (MQ=255)                                                                gggCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATAtt              >  2:331747/1‑81 (MQ=255)                                                                 ggCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATAtt              >  2:331757/1‑80 (MQ=255)                                                                 ggCGTCTGCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTg             <  1:331709/81‑1 (MQ=255)                                                                        gCACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCg       >  2:331755/1‑80 (MQ=255)                                                                         cACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGcc        <  2:331710/78‑1 (MQ=255)                                                                         cACGCGCCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCg       <  1:331711/79‑1 (MQ=255)                                                                            gcgcCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAg  <  2:331712/81‑1 (MQ=255)                                                                            gcgcCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAg  >  2:331751/1‑81 (MQ=255)                                                                               |                                                                              AAAGAAGTCTTTCGCCACGAAGTTTTCAATCTCTTCATCGCGGCGCACCACCAGCCCAAGCACGGGCGTCTGCACGCGTCCCACGGAAAGTACGCCCTGATAACCGGCATTGCGACCGAGAATGGTATACGCACGGGTCATATTGATGCCGTACAG  >  NC_000913/1846295‑1846450

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑