Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,868,114	T→C	Y403H (TAT→CAT)	yeaG →	protein kinase YeaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,868,114	0	T	C	100.0%	101.8 / NA	30	Y403H (TAT→CAT)	yeaG	protein kinase YeaG
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/12);  total (18/12)

GAAAACTCCAGCATTTATTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTC  >  NC_000913/1868036‑1868191                                                                               |                                                                              gAAAACTCCAGCATTTATTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCAt                                                                             <  1:334719/81‑1 (MQ=255)  aaaaCTCCAGCATTTATTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATc                                                                            <  1:334720/81‑1 (MQ=255)   aaaCTCCAGCATTTATTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCAt                                                                             >  2:334743/1‑79 (MQ=255)   aaaCTCCAGCATTTATTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCAt                                                                             >  2:334749/1‑79 (MQ=255)        ccAGCATTTATTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGaa                                                                       >  2:334752/1‑80 (MQ=255)          aGCATTTATTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAatat                                                                    >  1:334741/1‑81 (MQ=255)                 aTTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGAc                                                              >  1:334747/1‑80 (MQ=255)                    cAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACg                                                          >  2:334753/1‑81 (MQ=255)                        gATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCgg                                                      >  2:334751/1‑81 (MQ=255)                         aTGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGgt                                                     >  1:334766/1‑81 (MQ=255)                            cGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTc                                                  <  2:334721/81‑1 (MQ=255)                              ggTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGa                                                >  2:334761/1‑81 (MQ=255)                                tttATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGAt                                               >  2:334744/1‑80 (MQ=255)                                    tgatgGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAgg                                          <  1:334722/81‑1 (MQ=255)                                         gCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGa                                     <  1:334724/81‑1 (MQ=255)                                         gCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGa                                     <  1:334723/81‑1 (MQ=255)                                           gAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAAc                                   >  1:334748/1‑81 (MQ=255)                                           gAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAAc                                   >  2:334756/1‑81 (MQ=255)                                             aaGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACgg                                 <  2:334725/81‑1 (MQ=255)                                              aGTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGt                                >  1:334754/1‑81 (MQ=255)                                               gTCTGAAAGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTc                               >  2:334745/1‑81 (MQ=255)                                                      aGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGAc                        <  2:334726/81‑1 (MQ=255)                                                      aGACACCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGAc                        <  1:334727/81‑1 (MQ=255)                                                        acacCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGAcgc                      >  2:334759/1‑81 (MQ=255)                                                          acCGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGt                    >  1:334750/1‑81 (MQ=255)                                                            cGATCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGttt                  >  1:334758/1‑81 (MQ=255)                                                               tCCCAAAGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGc               <  2:334728/81‑1 (MQ=255)                                                                   aaaGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGttt           <  2:334729/81‑1 (MQ=255)                                                                     aGCCAAGTCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGttt           >  2:334763/1‑79 (MQ=255)                                                                            tCGCATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCctc  <  2:334730/81‑1 (MQ=255)                                                                               |                                                                              GAAAACTCCAGCATTTATTCAAAGATGCGGGTTTATGATGGCGAAAGTCTGAAAGACACCGATCCCAAAGCCAAGTCGTATCAGGAATATCGTGACTACGCCGGTGTCGATGAAGGGATGAACGGTCTGTCGACGCGTTTTGCGTTTAAGATCCTC  >  NC_000913/1868036‑1868191

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑