Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,878,986	A→G	intergenic (+38/+21)	yoaJ → / ← yeaQ	uncharacterized protein YoaJ/PF04226 family protein YeaQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,878,986	0	A	G	100.0%	102.2 / NA	30	intergenic (+38/+21)	yoaJ/yeaQ	uncharacterized protein YoaJ/PF04226 family protein YeaQ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (18/12);  total (18/12)

GATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAA  >  NC_000913/1878909‑1879062                                                                              |                                                                             gATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGcc                                                                           >  1:336307/1‑81 (MQ=255) gATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGcc                                                                           >  1:336302/1‑81 (MQ=255)  attattGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGTAAAGGCTGCCTGTGTGCGGCCt                                                                          >  1:336293/1‑81 (MQ=255)        gTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTtgtg                                                                     >  2:336304/1‑80 (MQ=255)             aCTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAAtt                                                                >  2:336297/1‑80 (MQ=255)             aCTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAAtt                                                                >  2:336296/1‑80 (MQ=255)              cTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAAttt                                                               <  1:336270/80‑1 (MQ=255)                    aCTGAGCTGGGATGGTGGCAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGtt                                                        <  1:336271/81‑1 (MQ=255)                        aGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAAc                                                     <  1:336272/80‑1 (MQ=255)                        aGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACt                                                    <  1:336273/81‑1 (MQ=255)                          cTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACttt                                                  <  2:336274/81‑1 (MQ=255)                                    ggtAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCt                                        >  1:336311/1‑81 (MQ=255)                                     gtAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTg                                       <  2:336275/81‑1 (MQ=255)                                      tAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGt                                      <  2:336276/81‑1 (MQ=255)                                       aaCGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTg                                       >  1:336306/1‑79 (MQ=255)                                       aaCGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTg                                       >  1:336310/1‑79 (MQ=255)                                       aaCGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTg                                       >  2:336301/1‑79 (MQ=255)                                       aaCGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTg                                       >  2:336303/1‑79 (MQ=255)                                            cACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATa                                 >  2:336316/1‑80 (MQ=255)                                                    aaaaaTAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCAc                         <  1:336278/80‑1 (MQ=255)                                                    aaaaaTAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACg                        <  1:336277/81‑1 (MQ=255)                                                       aaTAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGAc                      >  2:336308/1‑80 (MQ=255)                                                        aTAGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACaa                    >  2:336318/1‑81 (MQ=255)                                                          aGCAAAGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATc                  >  2:336319/1‑81 (MQ=255)                                                             aaaGGCTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCa               <  1:336279/81‑1 (MQ=255)                                                                  cTGCCTGTGTGCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAAt          <  2:336280/81‑1 (MQ=255)                                                                        gtgtgCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACgg     >  1:336322/1‑80 (MQ=255)                                                                          gtgCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCaa  >  2:336314/1‑81 (MQ=255)                                                                          gtgCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAACAGCACGACAATCGCACCAATAACGGCaa  >  1:336305/1‑81 (MQ=255)                                                                           tgCGGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCaa  <  1:336281/80‑1 (MQ=255)                                                                              |                                                                             GATTATTGTCGTACTCGGCACTGAGCTGGGATGGTGGTAACGTCACCTCTAAAAAATAGCAAAGGCTGCCTGTGTGCAGCCTTTGTGCAATTTAAGCGTTAACTTTTAATCTTCCTGTAGATAAATAGCACGACAATCGCACCAATAACGGCAA  >  NC_000913/1878909‑1879062

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑