Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,925,648	G→A	C70Y (TGT→TAT)	yobB →	putative carbon‑nitrogen hydrolase family protein YobB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,925,648	0	G	A	100.0%	83.0 / NA	24	C70Y (TGT→TAT)	yobB	putative carbon‑nitrogen hydrolase family protein YobB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/12);  total (12/12)

TTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGG  >  NC_000913/1925574‑1925723                                                                           |                                                                            ttctttACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGc                                                                       >  1:343735/1‑81 (MQ=255)       aCTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCaa                                                                  <  2:343696/80‑1 (MQ=255)                       tCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGAc                                                  >  1:343747/1‑80 (MQ=255)                        cgcgACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGAcc                                                 <  2:343697/80‑1 (MQ=255)                          cgACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACcat                                               >  1:343725/1‑80 (MQ=255)                           gACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACcatca                                             >  1:343742/1‑81 (MQ=255)                              gTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTg                                          >  1:343731/1‑81 (MQ=255)                                     cccTGCCCCATCCGATCTCTCACTGTTAGACCCGCTTTATTACGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCt                                   >  2:343732/2‑81 (MQ=255)                                      ccTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCtt                                  >  1:343721/1‑81 (MQ=255)                                        tGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTcc                                >  1:343738/1‑81 (MQ=255)                                         gCCCCACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTccc                               >  1:343723/1‑81 (MQ=255)                                          ccccACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTccc                               <  2:343698/80‑1 (MQ=255)                                          ccccACCCGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCg                              <  1:343699/81‑1 (MQ=255)                                                ccGATCTCTCACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAat                        <  2:343700/81‑1 (MQ=255)                                                    tctctcACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATaa                    >  1:343729/1‑81 (MQ=255)                                                        tcACTGTTAGACCGGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAatat                      <  1:343702/75‑1 (MQ=255)                                                        tcACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAAtata                     <  1:343701/76‑1 (MQ=255)                                                        tcACTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGAt                >  1:343724/1‑81 (MQ=255)                                                          aCTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGAt                <  2:343703/79‑1 (MQ=255)                                                          aCTGTTAGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCg              <  1:926120/81‑1 (MQ=255)                                                                aGACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTa         <  2:343704/80‑1 (MQ=255)                                                                 gACCCGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTAtt       >  1:343728/1‑81 (MQ=255)                                                                   cccGCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCg     <  1:343705/81‑1 (MQ=255)                                                                      gCTTTATTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTgg  <  1:343706/81‑1 (MQ=255)                                                                           |                                                                            TTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACTGTTAGACCCGCTTTGTTATGCCGCAACGACCTGGCGAATGACCATCATTGCCGGCCTTCCCGTTGAATATAACGATCGCTTTATTCGTGG  >  NC_000913/1925574‑1925723

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑