Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,928,777	A→G	D21D (GAT→GAC)	ptrB ←	oligopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,928,777	0	A	G	100.0%	109.5 / NA	32	D21D (GAT→GAC)	ptrB	oligopeptidase B
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/13);  total (19/13)

CCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTT  >  NC_000913/1928700‑1928851                                                                              |                                                                           ccGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTc                                                                          >  2:344258/1‑80 (MQ=255)  cGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGa                                                                        >  2:344260/1‑81 (MQ=255)   gTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACACGTATCGTCCCGCAGCCAGTAGTAATTGTCGAt                                                                       <  2:344218/81‑1 (MQ=255)       cTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATgcgc                                                                   >  1:344263/1‑81 (MQ=255)       cTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATgcgc                                                                   <  1:344219/81‑1 (MQ=255)             tCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATc                                                              <  2:344220/80‑1 (MQ=255)                tgttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCa                                                          >  1:344221/1‑81 (MQ=255)                 gttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCAt                                                         >  1:344256/1‑81 (MQ=255)                  ttgCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCAt                                                         >  1:344257/1‑80 (MQ=255)                     cAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGaa                                                      <  2:344222/80‑1 (MQ=255)                        gTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGt                                                  >  1:344245/1‑81 (MQ=255)                         tAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGt                                                  >  1:344249/1‑80 (MQ=255)                          aGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCa                                                >  1:344252/1‑81 (MQ=255)                             ccAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATgg                                             >  2:344259/1‑81 (MQ=255)                                ggACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGt                                          >  2:344261/1‑81 (MQ=255)                                         gCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgc                                 >  1:344254/1‑81 (MQ=255)                                         gCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgc                                 <  1:344223/81‑1 (MQ=255)                                          cTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcg                                <  2:344224/81‑1 (MQ=255)                                             agaACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggc                             >  2:344264/1‑81 (MQ=255)                                             agaACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggc                             <  2:344225/81‑1 (MQ=255)                                              gaACGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggcg                            <  2:344226/81‑1 (MQ=255)                                               aaCGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATgcgggcgg                           <  1:344227/81‑1 (MQ=255)                                                aCGCGTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGggcggc                          >  1:344266/1‑81 (MQ=255)                                                 cgcgTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCt                         >  1:344279/1‑81 (MQ=255)                                                    gTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtttt                      <  2:344229/81‑1 (MQ=255)                                                    gTATCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCtttt                      <  1:344228/81‑1 (MQ=255)                                                       tCGTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGt                   <  2:344230/81‑1 (MQ=255)                                                         gTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAg                 >  1:344277/1‑81 (MQ=255)                                                         gTCCCGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAg                 >  2:344267/1‑81 (MQ=255)                                                             cGCAGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATg             <  1:344231/81‑1 (MQ=255)                                                                aGCCAGTAGTAATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTa          >  2:344253/1‑81 (MQ=255)                                                                        gtaATTGTCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGttctt  >  1:344265/1‑81 (MQ=255)                                                                              |                                                                           CCGTAACTATTTTCTTGTTGCAGGTAGTCCAGGACTTCTGGCTGAGAACGCGTATCGTCCCGCAGCCAGTAGTAATTATCGATGCGCGTATCGCCATGAAGCGTCATGGCGTGGGGAATGCGGGCGGCTTTTGGTAGCATGTTATTGTTCTT  >  NC_000913/1928700‑1928851

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑