Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,931,057	T→C	I59I (ATT→ATC)	purT →	phosphoribosylglycinamide formyltransferase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,931,057	0	T	C	100.0%	58.5 / NA	18	I59I (ATT→ATC)	purT	phosphoribosylglycinamide formyltransferase 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/7);  total (11/7)

GAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCG  >  NC_000913/1930989‑1931123                                                                     |                                                                   gagGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGAt                                                        >  1:344632/1‑81 (MQ=255) gagGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGAt                                                        >  2:344624/1‑81 (MQ=255)          gCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCa                                               >  1:344611/1‑81 (MQ=255)               cGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACg                                          >  1:344634/1‑81 (MQ=255)                     cTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCgtgt                                    >  1:344625/1‑81 (MQ=255)                          ccGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTg                               <  1:344584/81‑1 (MQ=255)                           cGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTg                               >  2:344631/1‑80 (MQ=255)                               gCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTg                          >  2:344636/1‑81 (MQ=255)                                  ccAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGaa                       <  2:344585/81‑1 (MQ=255)                                  ccAGCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGaa                       <  2:344586/81‑1 (MQ=255)                                     gCCATGCATGTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGaaaaa                    >  2:344644/1‑81 (MQ=255)                                           catgTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACcaca               <  2:344587/80‑1 (MQ=255)                                            atgTCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACAt              >  2:344626/1‑80 (MQ=255)                                               tCGCGCATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATtat           <  2:344588/80‑1 (MQ=255)                                                 gcgcATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATc        <  1:344589/81‑1 (MQ=255)                                                    cATCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTg     >  1:344633/1‑81 (MQ=255)                                                      tCGCTCCCATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGcc   <  2:344590/81‑1 (MQ=255)                                                           cccATGTCATCAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCg  >  2:344637/1‑77 (MQ=255)                                                                     |                                                                   GAGGTGATTGCCGTCGATCGCTATGCCGACGCACCAGCCATGCATGTCGCGCATCGCTCCCATGTCATTAATATGCTTGATGGTGATGCATTACGCCGTGTGGTTGAACTGGAAAAACCACATTATATCGTGCCG  >  NC_000913/1930989‑1931123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑