Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	221,842	C→T	M268I (ATG→ATA)	metN ←	L‑methionine/D‑methionine ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	221,842	0	C	T	100.0%	130.2 / NA	36	M268I (ATG→ATA)	metN	L‑methionine/D‑methionine ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/18);  total (18/18)

TTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCG  >  NC_000913/221765‑221918                                                                              |                                                                             ttgttgACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTAt                                                                            <  1:52705/80‑1 (MQ=255)    ttgACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCgg                                                                         <  2:52706/80‑1 (MQ=255)    ttgACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCgg                                                                         >  1:52752/1‑80 (MQ=255)           tGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGcagt                                                                 >  2:52757/1‑81 (MQ=255)            gAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGcagt                                                                 <  1:52707/80‑1 (MQ=255)             aaaCGACGCGCGGTTTCAGAAAGCAGTGGGACATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGcagtc                                                                <  2:52708/80‑1 (MQ=255)                    gcgcgGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATg                                                        >  2:52751/1‑81 (MQ=255)                       cgGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCt                                                     >  2:52764/1‑81 (MQ=255)                       cgGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCt                                                     <  1:52709/81‑1 (MQ=255)                        ggTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTc                                                    >  2:52754/1‑81 (MQ=255)                        ggTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTc                                                    >  1:52758/1‑81 (MQ=255)                        ggTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTc                                                    <  2:52710/81‑1 (MQ=255)                          tttCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCg                                                  >  2:52770/1‑81 (MQ=255)                             cAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCt                                                <  1:52711/80‑1 (MQ=255)                               gAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTg                                              >  2:52753/1‑80 (MQ=255)                                aaaGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTg                                              >  2:52763/1‑79 (MQ=255)                                 aaGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAg                                           <  2:52712/81‑1 (MQ=255)                                   gCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGAc                                         <  2:52714/81‑1 (MQ=255)                                   gCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGAc                                         <  2:52713/81‑1 (MQ=255)                                    cAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACg                                        <  2:52715/81‑1 (MQ=255)                                     aGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGt                                       <  2:52716/81‑1 (MQ=255)                                      gTGGGGCATCGACCGATTGACCGGTAAACCCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGtt                                      >  1:52773/1‑81 (MQ=255)                                         gggCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTcc                                    <  2:52717/80‑1 (MQ=255)                                           gCATCGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTgg                                 <  2:52718/81‑1 (MQ=255)                                               cGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAAt                             >  1:52756/1‑81 (MQ=255)                                               cGACCGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAAt                             >  1:52749/1‑81 (MQ=255)                                                   cGATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTc                         >  1:52755/1‑81 (MQ=255)                                                    gATTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTcc                        >  2:52771/1‑81 (MQ=255)                                                     aTTGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCg                       <  1:52719/81‑1 (MQ=255)                                                      ttGACCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCgg                      >  2:52747/1‑81 (MQ=255)                                                         aCCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGat                   <  2:52720/81‑1 (MQ=255)                                                         aCCGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGat                   <  2:52721/81‑1 (MQ=255)                                                          ccGGTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGata                  >  1:52760/1‑81 (MQ=255)                                                             gTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATc                >  1:52818/1‑80 (MQ=255)                                                             gTAAACTCCAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATc                >  2:52765/1‑80 (MQ=255)                                                                     cAGACGCAGTATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGc         <  1:52722/79‑1 (MQ=255)                                                                          gcagtaTCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCg  <  2:52723/81‑1 (MQ=255)                                                                              |                                                                             TTGTTGACGTTGAAACGACGCGCGGTTTCAGAAAGCAGTGGGGCATCGACCGATTGACCGGTAAACTCCAGACGCAGCATCGGCACGCAGTCAGTAAATGGCTCCGCTTGCAGACGTTCCTGGTAATCTTCCGGGATATCCAGATGCAGGGTCG  >  NC_000913/221765‑221918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑